家蚕分子遗传操作技术研究进展  

Advances in Molecular Genetic Manipulation Techniques in the Silkworm,Bombyx mori

作　　者：龙定沛[1] 邹云龙 汪林 杨镇霖 向仲怀[1] 代方银[1] LONG Dingpei;ZOU Yunlong;WANG Lin;YANG Zhenlin;XIANG Zhonghuai;DAI Fangyin(State Key Laboratoryof Resource Insects/Key Laboratory of Sericultural Biology and Genetic Breeding,Ministry of Agriculture and Rural Affairs/Institute of Sericulture and Systems Biology,Southwest University,Chongqing 400715,China)

机构地区：[1]资源昆虫高效养殖与利用全国重点实验室/农业农村部蚕桑生物学与遗传育种重点实验室/西南大学蚕学与系统生物学研究所,重庆400715

出　　处：《蚕学通讯》2024年第3期63-73,共11页Newsletter of Sericultural Science

基　　金：重庆英才·青年拔尖人才项目(CSTC2022YCJH-BGZXM0019);重庆市自然科学基金项目(CSTB2023NSCQ-MSX0506,cstc2021jcyj-cxtt0005);重庆市在渝院士牵头科技创新引导专项(CSTB2023YSZX-JCX0003)。

摘　　要：家蚕的分子遗传操作是鉴定基因功能、开展分子育种和种质材料创新等研究的重要平台技术,对于推动家蚕实验动物模式化,推进蚕业科学深度融入生命科学和促进传统蚕丝业高质量发展具有重要支撑作用。近年来,家蚕分子遗传操作技术取得了显著进展,包括转基因技术、基因组靶向编辑技术、时空特异性表达技术及其他辅助分子遗传操作技术均得到了广泛地应用,使得家蚕在农业、纺织业以及医学与材料科学等学科领域的基础和应用研究取得了一系列重要成果。本文综述了家蚕分子遗传操作技术的建立、发展现状和面临的问题,旨在为家蚕分子遗传操作技术在现代分子育种及生物材料开发等研究中的应用提供参考和指导。Molecular genetic manipulation in silkworms serves as a crucial platform for identifying gene functions, advancing molecular breeding, and innovating material development. It plays an essential role inpromoting the establishment of the silkworm as a model organism, integrating sericulture research moredeeply into life sciences, and supporting the continuous growth of the traditional silk industry. In recentyears, there have been significant advancements in silkworm molecular genetic manipulation techniques,including transgenic technology, genome-targeted editing, spatio-temporal specific expression systems,and other auxiliary molecular tools. These techniques have been widely applied and developed, yielding importantbreakthroughs in various fields such as agriculture, textiles, medicine, and materials science. Thisreview summarizes the establishment, current developments, and challenges of silkworm molecular geneticmanipulation techniques, aiming to provide insights and guidance for furthering their application in modernsilkworm molecular breeding research.

关 键 词：家蚕 分子遗传操作 转基因 基因组编辑 基因表达调控 

分 类 号：S881.26[农业科学—特种经济动物饲养]