克氏原螯虾湖北7个养殖群体遗传多样性的SSR分析  

Genetic diversity analysis of the seven aquaculture populations of Procambarus clarkia in Hubei Province based on SSR markers

作　　者：崔学海 沙航 曹继增 郜卫华[2] 梁宏伟[1] CUI Xuehai;SHA Hang;CAO Jizeng;GAO Weihua;LIANG Hongwei(Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;College of Animal Science and Technology,Yangtze University,Jingzhou 434025,Hubei,China)

机构地区：[1]中国水产科学研究院长江水产研究所,武汉430223 [2]长江大学动物科学学院,湖北荆州434025

出　　处：《淡水渔业》2024年第6期79-86,共8页Freshwater Fisheries

基　　金：中国水产科学研究院长江水产研究所基本科研业务费专项(YFI202203);中国水产科学研究院院级基本科研业务费专项(2022XT0103)。

摘　　要：为了解湖北主要养殖地区克氏原螯虾(Procambarus clarkii)群体遗传多样性本底水平,以湖北鄂州(EZ)、公安(GA)、洪湖(HH)、监利(JLi)、江陵(JL)、潜江(QJ)、石首(SS)7个养殖群体为研究对象,利用10个克氏原螯虾微卫星标记进行遗传多样性和遗传结构研究。结果显示,克氏原螯虾鄂州群体遗传多样性最高,洪湖群体遗传多样性最低。93.58%的群体遗传变异来自群体内。7个群体间遗传分化系数(F_(st))表明,各群体之间均未出现高度的遗传分化,不同群体之间存在着基因交流,洪湖群体和江陵群体之间基因交流最为广泛。基于Nei’s距离构建的UPGMA系统发育树显示,7个群体分为3支,即鄂州(EZ)、公安(GA)、潜江(QJ)群体聚为一支,洪湖(HH)、监利(JLi)、江陵(JL)群体聚为一支,石首(SS)群体单独为一支。经Structure软件分析发现,7个克氏原螯虾群体最有可能划分为5个亚群。本研究的7个克氏原螯虾养殖群体具有中等的遗传多样性,鄂州群体遗传多样性最高,具有相对较高的选育潜力。Genetic diversity and genetic structure of seven farming populations of Procambarus clarkii from Ezhou(EZ),Gongan(GA),Honghu(HH),Jianli(JLi),Jiangling(JL),Qianjiang(QJ)and Shishou(SS)were studied using ten microsatellite markers to understand the genetic background of P.clarkii populations in the main farming areas in Hubei province.Analysis of molecular variationce(AMOVA)revealed that about 6.42%of genetic variation was derived from inter-populations,and 93.58%of genetic variation from intra-populations.The genetic differentiation coefficient(F_(st))indicated no high genetic differentiation among the populations,and there was the most extensive gene flow between Honghu and Jiangling.The UPGMA phylogenetic tree constructed based on the Nei's distance shows that the seven populations are divided into three groups,Ezhou,Gongan and Qianjiang populations as one group,Honghu,Jianli and Jiangling populations as another group,and the Shishou population as its group,respectively.The analysis revealed that the seven crayfish populations are most likely divided into five sub-populations.The seven Procambarus clarkii populations in this study have moderate genetic diversity,with the Ezhou population having the highest genetic diversity and potential for selection and breeding.

关 键 词：克氏原螯虾(Procambarus clarkii) 湖北养殖群体 微卫星 遗传多样性 遗传结构 

分 类 号：S966.12[农业科学—水产养殖]