Understanding mechanisms for differential salinity tissue tolerance between quinoa and spinach:Zooming on ROS-inducible ion channels  

作　　者：Mohsin Tanveer Lei Wang Liping Huang Meixue Zhou Zhong-Hua Chen Sergey Shabala 

机构地区：[1]State Key Laboratory of Desert and Oasis Ecology,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830000,Xinjiang,China [2]International Research Centre for Environmental Membrane Biology,Foshan University,Foshan 528000,Guangdong,China [3]Tasmanian Institute of Agriculture,University of Tasmania,Hobart,TAS 7005,Australia [4]School of Science,Western Sydney University,Penrith,NSW 2751,Australia [5]Hawkesbury Institute for the Environment,Western Sydney University,Penrith,NSW 2751,Australia [6]School of Biological Sciences,University of Western Australia,Perth,WA 6009,Australia

出　　处：《The Crop Journal》2024年第5期1357-1368,共12页作物学报（英文版）

基　　金：supported by the Grassland Ecological Restoration and Management Project of General Grassland Station of Xinjiang (XJCYZZ202102)。

摘　　要：Soil salinity is a wo rldwide issue and a major threat to global food security.Salinity tolerance is a complex mechanism that is conferred by numerous molecular,physiological,and biochemical traits.Of critical importance are plant's ability to regulate redox balance without compromising reactive oxygen species(ROS)signalling and maintain cytosolic ion homeostasis.In this study,the mechanistic basis of H^(+) retention ability in leaf mesophyll(an important but highly sensitive plant tissue)was compared between halophytic quinoa and glycophytic spinach.Phenotypic data showed quinoa outperformed spinach under 100 to 500 mmol L^(-1) NaCl salinity.The major difference behind this differential salinity sensitivity was a differential H^(+) uptake in leaf mesophyll.Electrophysiological and molecular experiments revealed that a superior ability of mesophyll H^(+) retention in quinoa was conferred by three complementary mechanisms:(ⅰ)an intrinsically lower H^(+)-ATPase activity in quinoa(potentially as an energy saving strategy);(ⅱ)reduced sensitivity of H^(+) transporters to ROS;and(ⅲ)increased sensitivity of ROS-inducible Ca^(2+)-permeable channels,Moreover,the sensitivity of H^(+)-transport systems to ROS was further examined in NaCl-acclimated quinoa and spinach plants.The key factors differentiating between H^(+) retention in acclimated leaf mesophyll was associated with the reduced sensitivity and gene expression of H^(+)-permeable outward rectifying channel(GORK),Arabidopsis potassium transporter 1(AKT1),and high affinity potassium transporter 5(HAK5)to additional NaCl and ROS stress,along with the upregulation of ROS scavenging system.Taken together,our results showed that the tissue-specific and ROS-specific regulation of H^(+) retention are important for conferring salinity tolerant at least in the halophyte quinoa.

关 键 词：ACCLIMATION Membrane potential ROS signalling Leaf mesophyll Chenopodium quinoa Spinacia oleracea 

分 类 号：S519[农业科学—作物学] S636.1