Photo-responsive Carboxymethyl Chitosan/Laponite Hydrogel as a Potential Spinal Cord Injury Scaffold:Characterization and Cytocompatibility Study  

作　　者：Jayanti Parajuli LI Yongtao CHANG Likun YE Liyuan HAN Yingchao YIN Yixia 殷义霞

机构地区：[1]State Key Laboratory of Advanced Technology for Material Synthesis and Processing,Wuhan University of Technology,Wuhan 430070,China [2]Biomedical Materials and Engineering Research Center of Hubei Province,Wuhan University of Technology,Wuhan 430070,China

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2024年第6期1628-1636,共9页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the Natural Science Foundation of Hubei Province(No.2022CFB386)。

摘　　要：We synthesized photo-responsive carboxymethyl chitosan(CMC-MA)via free radical polymerization and utilized nanoclay laponite(LAP)as an inorganic crosslinking agent to develop an injectable and 3D-printable CMC-MA/LAP hydrogel.We determined the optimal ratio of 2.5 w/v%CMC-MA/7.5 w/v%LAP based on injection molding,compression modulus,swelling properties,rheological properties,and 3D printing properties of the hydrogel system.In-vitro cytocompatibility experiments showed that both CMC-MA and CMC-MA/LAP hydrogel had no inhibitory effect on cell proliferation and can promote cell growth when cultured on the surface of the hydrogel matrix.Moreover,the hydrogel containing LAP particles significantly facilitated cell adhesion(>60%)compared with the hydrogel without LAP(20%).Our findings demonstrate that the CMC-MA/LAP hydrogel has great potential for tissue repair in neural tissue engineering.

关 键 词：neural injectable hydrogels spinal cord injury CMC-MA/LAP hydrogel CYTOCOMPATIBILITY 

分 类 号：TQ427.26[化学工程] R318.08[医药卫生—生物医学工程]