电针联合奥美拉唑保护NSAIDs大鼠胃溃疡的增效作用评价  

Evaluation of synergistic effect of electroacupuncture combined with Omeprazole on gastric ulcer protection in NSAIDs rats

作　　者：卢登杰 张攀攀 林静瑜 周凡 Lu Dengjie;Zhang Panpan;Lin Jingyu;Zhou Fan(Fujian Academy of Chinese Medical Sciences,Fuzhou Fujian 350003;Acupuncture College of Fujian University of Traditional Chinese Medicine,Fuzhou Fujian 350108)

机构地区：[1]福建省中医药科学院,福建福州350003 [2]福建中医药大学针灸学院,福建福州350108

出　　处：《山西中医药大学学报》2024年第9期986-991,1032,共7页Journal of Shanxi University of Chinese Medicine

基　　金：福建省自然科学基金资助项目(2021J01918,2018J01853)。

摘　　要：目的:评价电针足三里与不同剂量奥美拉唑联用保护吲哚美辛所致大鼠胃溃疡的增效作用。方法:48只雄性SD大鼠随机分为8组:空白组、模型组、奥美拉唑低剂量组(5 mg/kg,Omeprazole L)、奥美拉唑中剂量组(10 mg/kg,Omeprazole M)、奥美拉唑高剂量组(20 mg/kg,Omeprazole H)、电针联合奥美拉唑低剂量组(E+Omeprazole L)、电针联合奥美拉唑中剂量组(E+Omeprazole M)、电针联合奥美拉唑高剂量组(E+Omeprazole H),每组6只;末次给药前1 h,除空白组外各组以吲哚美辛(40 mg/kg,腹腔注射)制备胃溃疡模型;造模后7 h取材,观察胃黏膜大体外观及胃、肠组织病理形态,对胃黏膜外观及病理损伤作评分。结果:模型组大鼠胃黏膜损伤严重,黏膜表面部分变薄,见点线状出血或糜烂、溃疡灶,病理病变以黏膜层出现红细胞及上皮细胞变性缺损为主。小肠黏膜病理病变呈轻度损伤,见绒毛纹状边缘脱落消失,上皮细胞空泡化。(1)与模型组比较,干预各组胃黏膜大体外观及病理病变改善,胃黏膜损伤评分降低(P<0.05,P<0.01)。除Omeprazole L组外,其余各组病理评分均较模型组降低(Omeprazole H组P=0.08,余P<0.01)。以Omeprazole M组、E+Omeprazole M组、E+Omeprazole H组损伤抑制率最高;(2)Omeprazole 3个剂量组间比较:与Omeprazole L组、Omeprazole H组比较,Omeprazole M组胃黏膜大体外观及病理病变减轻,胃黏膜损伤分数及病理评分降低(P<0.01,P<0.05);(3)E+Omeprazole 3个剂量组间比较:与E+Omeprazole L组比较,E+Omeprazole M组、E+Omeprazole H组胃黏膜大体外观损伤減轻,损伤分数明显降低(P<0.05,P<0.01);(4)同剂量Omeprazole组与E+Omeprazole组间比较:与相同剂量Omeprazole组比较,E+Omeprazole L组、E+Omeprazole H组胃黏膜大体外观、病理病变减轻,损伤分数降低(P<0.05,P<0.01),病理评分呈降低趋势(P=0.1,P=0.08);(5)模型组小肠黏膜轻度损伤,绒毛纹状边缘部分消失脱落,上皮细胞浆空泡。与模型组比�Objective:To evaluate the synergistic effect of electroacupuncture at Zusanli acupoint combined with different doses of Omeprazole on the protection of Indomethacin-induced gastric ulcer in rats.Methods:48 male SD rats were randomly divided into 8 groups:blank group,model group,low-dose Omeprazole(5 mg/kg,Omeprazole L)group,medium-dose Omeprazole(10 mg/kg,Omeprazole M)group,high-dose Omeprazole(20 mg/kg,Omeprazole H)group,electroacupuncture combined with low-dose Omeprazole(E+Omeprazole L)group,electroacupuncture combined with medium-dose Omeprazole(E+Omeprazole M)group,and electroacupuncture combined with high-dose Omeprazole(E+Omeprazole H)group,with 6 rats in each group.At 1 h before the last administration,the gastric ulcer model was prepared by Indomethacin(40 mg/kg intraperitoneally injected)in all groups except the blank group.The gross appearance of gastric mucosa and pathological morphology of gastric and intestinal tissues were observed 7 h after modeling,and the appearance and pathological damage of gastric mucosa were scored.Results:In the model group,the gastric mucosa was seriously damaged,and the mucosal surface was partially thinner,with linear bleeding,erosion or foci of ulcer.The pathological changes were mainly red cell and epithelial cell degeneration defect in the mucosal layer.The pathological changes of small intestinal mucosal lesions were slightly damaged,and the villous striate margin was shed and the epithelial cells vacuolated.(1)Compared with the model group,the gross appearance and pathological changes of gastric mucosa in each intervention group were improved,and the gastric mucosal injury score was decreased(P<0.05,P<0.01).Except for Omeprazole L group,the pathological scores of other groups were lower than those of model group(Omeprazole H group P=0.08,the rest P<0.01).The damage inhibition rate was the highest in the Omeprazole M group,E+Omeprazole M group and E+Omeprazole H group.(2)Comparison among the three doses groups of Omeprazole:compared with Omeprazole L group and Omepra

关 键 词：NSAIDs胃病 针药结合 足三里 奥美拉唑 增效作用 

分 类 号：R245[医药卫生—针灸推拿学]