氯胁迫下水凝胶用量对烟苗生长发育和抗氧化酶活性的影响  

Effects of Hydrogel on Growth and Antioxidant Enzymes Activities of Tobacco Seedlings under Chlorine Stress

作　　者：高子婷 罗菲 杨建新 刘玲玲 刘新源[2] 夏茂林 李俊营 许跃奇 姬小明[1] Gao Ziting;Luo Fei;Yang Jianxin;Liu Lingling;Liu Xinyuan;Xia Maolin;Li Junying;Xu Yueqi;Ji Xiaoming(College of Tobacco,Henan Agricultural University,Zhengzhou 450002,China;Sanmenxia Company of Henan Tobacco Corporation,Sanmenxia 472000,China;Fujian Jinmin Reclaimed Tobacco Development Co.,Ltd.,Fuzhou 350600,China;Pingdingshan Company of Henan Tobacco Corporation,Pingdingshan 467000,China)

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]河南省烟草公司三门峡市公司,河南三门峡472000 [3]福建金闽再造烟叶发展有限公司,福建福州350600 [4]河南省烟草公司平顶山市公司,河南平顶山467000

出　　处：《山东农业科学》2024年第10期80-85,共6页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(32300342);国家重点研发计划项目(2017YFD0200808);河南省烟草公司平顶山市公司项目(PYKJ202401);河南省烟草公司三门峡市公司项目(2022411200200004x)。

摘　　要：为缓解氯胁迫对烟苗生长发育的不利影响,本试验以水凝胶为材料,设置5个处理即CK(不施KCl和水凝胶)、CK1(1.15 g KCl/盆+0 g水凝胶)、T1(1.15 g KCl/盆+1 g水凝胶/盆)、T2(1.15 g KCl/盆+3 g水凝胶/盆)和T3(1.15 g KCl/盆+5 g水凝胶/盆),研究氯胁迫下施用水凝胶对烟苗生长发育、抗氧化系统和常规化学成分的影响。结果表明,随着水凝胶用量增加,烟苗氯含量呈下降趋势,T2、T3处理烟苗氯含量较CK1分别降低21.35%和30.62%;T2处理烟苗地上部地下部鲜重、地上部地下部干重较CK1分别提高16.90%、20.29%、20.00%和30.93%,T3处理烟苗地上部地下部鲜重、地上部地下部干重较CK1分别提高27.70%、22.46%、30.77%和37.11%;T2、T3处理烟苗光合速率较CK1分别提高29.23%、41.78%;T2处理烟苗过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量较CK1分别降低19.30%、22.11%、12.98%和13.25%,T3处理烟苗CAT、POD、SOD活性以及MDA含量较CK1分别降低19.91%、21.59%、21.13%和20.33%;T2、T3处理有效提高氯胁迫下烟苗烟碱、总糖、还原糖含量和钾氯比。综上可见,施用水凝胶可有效缓解氯胁迫对烟苗的不利影响,促进烟苗生长发育。To mitigate the adverse effects of chlorine stress on growth and development of tobacco seedlings,five treatments namely CK(no KCl and hydrogel),CK1(1.15 g KCl per pot and no hydrogel),T1(1.15 g KCl+1 g hydrogel per pot),T2(1.15 g KCl+3 g hydrogel per pot)and T3(1.15 g KCl+5 g hydrogel per pot)were set in this experiment to study the effects of hydrogel application on growth and development,antioxidant system and routine chemical constituents of tobacco seedlings under chlorine stress.The results showed that the chlorine content in tobacco seedlings had a decreasing trend with the increase of hydrogel application rate,and that decreased by 21.35%and 30.62%under T2 and T3 treatment compared to CK1.The fresh weight of aboveground part,the fresh weight of underground part,the dry weight of aboveground part and the dry weight of underground part under T2 treatment increased by 16.90%,20.29%,20.00%and 30.93%respectively compared to CK1,while those increased by 27.70%,22.46%,30.77%and 37.11%under T3 treatment.T2 and T3 treatments increased the photosynthetic rate of tobacco seedlings by 29.23%and 41.78%respectively compared to CK1.T2 treatment decreased the catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD)activities and the malondialdehyde(MDA)content by 19.30%,22.11%,12.98%and 13.25%,respectively compared to CK1,while T3 treatment decreased those by 19.91%,21.59%,21.13%and 20.33%respectively.T2 and T3 treatments effectively improved the content of nicotine,total sugar,reducing sugar and ratio of potassium to chlorine in tobacco seedlings under chlorine stress.In conclusion,the application of hydrogel could effectively alleviate the adverse effects of chlorine stress on tobacco seedlings,and promote their growth and development.

关 键 词：水凝胶 氯胁迫 烟苗 生长发育 抗氧化酶活性 

分 类 号：S572[农业科学—烟草工业]