芍药地上部分提取物提取工艺及其抗氧化活性的研究  

Study on Extraction Technology and Antioxidant Activity of The Extracts from The Above-Ground Parts of Paeonia lactiflora pall

作　　者：王明华 豆佳红 李云 李俊[2] 史万玉 李秀梅[2] WANG Minghua;DOU Jiahong;LI Yun;LI Jun;SHI Wanyu;LI Xiumei(Traditional Chinese Veterinary Medicine,College of Veterinary Medicine,Hebei Agricultural University,Hebei Baoding 071000,China;Institute of Feed Research of Chinese Academy of Agricultural Sciences,Beijing 100081,China;Shijiazhuang Animal Products and Veterinary Medicine Feed Quality Testing Center,Hebei Shijiazhuang 050000,China)

机构地区：[1]河北农业大学动物医学院中兽医学院,河北保定071000 [2]中国农业科学院饲料研究所,北京100081 [3]石家庄市畜产品和兽药饲料质量检测中心,河北石家庄050000

出　　处：《饲料工业》2024年第21期139-145,共7页Feed Industry

摘　　要：研究旨在以芍药地上部分为原料,采用单因素试验考察料液比、超声时间、超声温度、超声功率、乙醇浓度对芍药地上部分提取物抗氧化能力和提取率的影响。选取三因素三水平,采用正交试验优化芍药地上部分抗氧化提取物提取工艺,并分析各因素显著性。结果表明,芍药地上部分提取物适宜提取工艺为乙醇浓度50%、料液比1∶30 g/mL、提取功率500 W、提取时间25 min、提取温度55℃,在此条件下芍药地上部分提取物总抗氧化能力为(2.99±0.06)mmol/g,DPPH自由基清除率为(34.47±0.51)%,提取率可达到(25.26±0.22)%。优选的提取工艺稳定可行,且提取效率较高,此研究为芍药地上部分应用奠定基础。This experiment was conducted to investigate the effect of material-liquid ratio,ultrasound time,ultrasound temperature,ultrasound power,and ethanol concentration on the antioxidant capacity and extraction rate of the extracts from the above-ground parts of Paeonia lactiflora pall(PL)using a one-way experimental design.Three factors and three levels were selected to optimize the extraction pro⁃cess of antioxidant extract of above-ground part of PL,which used orthogonal experiment,and then the significance and interaction of each factor were analyzed.The results show that the suitable extraction process of PLE is as follows:ethanol concentration of 50%,material-liquid ratio of 1∶30 g/mL,extrac⁃tion power of 500 W,extraction time of 25 min,and extraction temperature of 55℃.Under these condi⁃tions,the total antioxidant capacity and DPPH free radical scavenging rate are(2.99±0.06)mmol/g and(34.47±0.51)%,and the extraction rate is(25.26±0.22)%.This study lays a foundation for the application of the above-ground part of PL.

关 键 词：芍药地上部分 超声提取 单因素试验 正交试验 抗氧化 

分 类 号：S816.7[农业科学—饲料科学]