m^(6)A修饰在病毒感染宿主细胞中的调节作用  

The Regulatory Role of m^(6)A Modification in Viral Infection of Host Cells

作　　者：夏月平 黄芬[1] XIA Yue-Ping;HUANG Fen(Faculty of Life Science and Technology&Medical School,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院&医学院,昆明650500

出　　处：《中国生物化学与分子生物学报》2024年第10期1362-1373,共12页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助项目(No.82260396);云南省自然科学基金资助项目(No.202401AS070057)资助。

摘　　要：N^(6)-甲基腺苷(N^(6)-methyladenosine,m^(6)A)是指RNA分子腺嘌呤第6位氮原子上发生的甲基化修饰,是信使RNA(mRNA)和非编码RNA(ncRNA)中最常见的转录后修饰。m^(6)A修饰在RNA循环的所有阶段,包括RNA稳定、剪接、核输出、折叠、翻译和降解等过程中发挥重要作用,这一过程需甲基转移酶(writers)、去甲基酶(erasers)和m^(6)A阅读蛋白(readers)的参与。随着RNA高通量测序技术的不断发展,m^(6)A修饰参与病毒与宿主互作中的研究不断涌现。研究表明m^(6)A修饰发生在多种RNA病毒中,影响病毒感染、复制及子代病毒粒子的生成。病毒也可通过改变宿主细胞转录物组的m^(6)A修饰影响病毒的感染性或宿主对病毒的抵抗性。本文对呼吸道病毒、反转录病毒、疱疹病毒等感染宿主细胞造成的m^(6)A修饰进行概述,并针对m^(6)A修饰对病毒的复制及对宿主免疫反应的调节作用进行综述,为了解病毒与宿主互作机制研究及抗病毒药物筛选供理论基础。N^(6)-methyladenosine(m^(6)A)is a methylation modification on the 6th nitrogen atom of the RNA molecule adenine,and is the most common post-transcriptional modification in messenger RNA(mRNA)and non-coding RNA(ncRNA).m^(6)A modification plays a crucial role in all stage of RNA cycle,including RNA stabilization,splicing,nuclear export,folding,translation and degradation.m^(6)A modification requires the participation of methyltransferase(writers),demethylase(erasers)and m^(6)A readers.The important roles of m^(6)A modification involved in virus-host interactions have been largely explored based on the rapid development of high-throughput RNA sequencing technology.Studies have shown that m^(6)A modification participates in a variety of viral RNAs,and is associated with viral entrance,replication and progeny virion release.Viruses can also alter host transcriptome by m^(6)A modification to impact viral infectivity or host resistance.This review explored the role of m^(6)A modification in respiratory virus,retrovirus and herpes virus,etc.infected cells,and the regulatory effects of m^(6)A modifications on viral replication and host immune response.It will help to further clarify the mechanisms of virus-host interactions and benefit the development of antiviral drugs.

关 键 词：N^(6)-methyladenosine(m^(6)A) m^(6)A甲基转移酶 m^(6)A去甲基酶 m^(6)A阅读蛋白 病毒 

分 类 号：Q939.4[生物学—微生物学]