尖孢镰刀菌古巴专化型铜伴侣蛋白FocCCS1的功能分析  

Functional analysis on copper chaperone FocCCS1 in Fusarium oxysporum f.sp.cubense

作　　者：邓火兵 尤海霞 黄梅梅 尹凡 杨金涛 云英子 DENG Huobing;YOU Haixia;HUANG Meimei;YIN Fan;YANG Jintao;YUN Yingzi(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学植物保护学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2024年第6期732-741,共10页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金面上项目(32272516)。

摘　　要：【目的】研究尖孢镰刀菌古巴专化型(Foc)铜伴侣蛋白FocCCS1的生物学功能,为香蕉枯萎病的绿色防控提供参考。【方法】利用MEGA-X软件构建FocCCS1基因的遗传进化树;通过基因敲除技术从Foc 4号热带生理小种(FocTR4)中敲除FocCCS1,构建基因敲除突变体和回补菌株,研究该基因对Foc生长、产孢、环境胁迫响应、致病性、菌丝穿透和黏附及镰刀菌酸合成等方面的调控作用。【结果】FocCCS1基因长度为879 bp,编码292个氨基酸,包含重金属ATP酶(HMA)和Sod_Cu结构域,其与轮枝镰刀菌FvCCS1亲缘关系最近。表型分析结果显示,FocCCS1参与调控Foc菌丝的生长、分生孢子的产生和萌发、对Cu2+胁迫和氧化胁迫的应答以及致病性,并且影响菌丝的黏附能力和镰刀菌酸的合成。【结论】FocCCS1是参与调控Foc致病性的相关基因,菌丝的黏附能力和镰刀菌酸的合成量下降可能是导致该菌致病性减弱的主要原因。【Objective】The biological function of Fusarium oxysporum f.sp.cubense(Foc)copper chaperone protein(FocCCS1)was investigated to provide a reference for the green prevention and control of banana wilt disease.【Method】The genetic evolutionary tree of FocCCS1 gene was constructed by MEGA-X software.Then the FocCCS1 knockout mutant of Foc No.4 tropical physiological race(FocTR4)and its complement strain were constructed by knockout technology to study the effects of FocCCS1 on the growth,sporulation,response to environmental stresses,pathogenicity,mycelial penetration and adherence,and fusaric acid synthesis of Foc.【Result】The FocCCS1 gene is 879 bp in length,and encodes 292 amino acids;it contains HMA and Sod_Cu domains,and is most closely related to FvCCS1 of F.verticillium.Phenotypic analysis showed that FocCCS1 was involved in the regulation of mycelial growth,conidial production and germination,response to Cu2+and oxidative stress,and pathogenicity of Foc,and affected mycelial adhesion and fusaric acid synthesis.【Conclusion】FocCCS1 is involved in the regulation of Foc pathogenicity-related genes,and the decrease in mycelial adhesion ability as well as the synthesis of fusaric acid may be the main reason for its weakened pathogenicity.

关 键 词：超氧化物歧化酶铜伴侣 基因敲除 营养生长 致病性 Cu2+响应 

分 类 号：S432.4[农业科学—植物病理学]