基于UPLC-Q-TOF/MS技术的枳实大鼠血清药物化学研究  

Study on Rat Serum Pharmacochemistry of Fructus Aurantii Immaturus Based on UPLC-Q-TOF/MS Method

作　　者：陈思琦 肖小武 郑洋滨 陈伟康 罗跃华 刘地发 CHEN Si-qi;XIAO Xiao-wu;ZHENG Yang-bin;CHEN Wei-kang;LUO Yue-hua;LIU Di-fa(Jiangxi Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Chinese Patent Medicine,Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Jiangxi Nanchang 330029,China;School of Pharmacy,Jiangxi Medical College,Nanchang University,Jiangxi Nanchang 330006,China;Jiangxi Qingfeng Pharmaceutical Industry Co.,Ltd.,Jiangxi Ganzhou 341000,China)

机构地区：[1]江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品医疗器械质量工程研究中心,江西南昌330046 [2]南昌大学江西医学院药学院,江西南昌330006 [3]江西青峰药业有限公司,江西赣州341000

出　　处：《中国药物评价》2024年第5期388-397,共10页Chinese Journal of Drug Evaluation

基　　金：江西省重点研发计划项目(20212BBG73025);江西省药品监督管理局科研项目(2021KY44);江西省主要学科学术和技术带头人培养计划-领军人才项目(20225BCJ22018)。

摘　　要：目的:运用血清药物化学方法分析大鼠口服枳实后的入血成分,初步阐明其可能的药效物质基础。方法:采用Agilent ZORBAX SB-C_(18)(3.0 mm×100 mm,1.8μm)色谱柱,以0.1%甲酸水(A)-乙腈(B)为流动相梯度洗脱,飞行时间质谱作为检测器分析样品。通过比较枳实提取物、空白血浆以及给药血浆的图谱差异,根据保留时间、精确相对分子质量、二级质谱裂解碎片及代谢产物的中性丢失等,鉴定大鼠灌胃给予枳实提取物后的血中原形成分及其代谢产物。结果:在给药后的大鼠血清中分析表征了21个入血成分,其中15个原形成分、6个代谢产物。结论:借助UPLC-Q-TOF/MS方法鉴定了大鼠口服枳实后血清中的化学成分,初步分析了枳实的入血成分,为阐明枳实质量标志物提供了理论依据。Objective:To analyze the blood components of rats after oral administration of the Fructus Aurantii Immaturus extract by the serum pharmacochemistry method to elucidated the effective constituents of Fructus Aurantii Immaturus.Methods:The samples were analyzed on Agilent ZORBAX SB-C_(18)(3.0 mm×100 mm,1.8μm)column with 0.1%formic acid water(A)-acetonitrile(B)as mobile phase gradient elution and time-of-flight mass spectrometry as detector..Based on the retention time,precise molecular weight,secondary mass spectrometry fragmentation and neutral loss of metabolites,compared with the difference of profiles of Fructus Aurantii Immaturus extract,blank serum and administered serum,and identified the prototypes and metabolites in blood of rats after administration of Fructus Aurantii Immaturus extract.Results:21 components were analyzed and characterized in the rat serum after administration,including 15 prototypes and 6 metabolites.Conclusion:The chemical components in serum of Fructus Aurantii Immaturus were identified by UPLC-Q-TOF/MS method,the chemical components in the rats serum after oral administration of the Fructus Aurantii Immaturus extract were preliminarily identified,and it could provided theoretical basis for definition the Q-Marker of Fructus Aurantii Immaturus.

关 键 词：枳实 血清药物化学 UPLC-Q-TOF/MS 原形成分 代谢产物 

分 类 号：R284.1[医药卫生—中药学] R965[医药卫生—中医学]