5株H6N3亚型禽流感病毒的分子特征及遗传演化分析  

Molecular Characterization and Phylogenetic Analysis of 5 Strains of H6N3 Subtype Avian Influenza Viruse

作　　者：陈源 崔鹏飞[1] 施建忠[1] 邓国华[1] 陈化兰[1] CHEN Yuan;CUI Pengfei;SHI Jianzhong;DENG Guohua*;CHEN Hualan(National Poultry Laboratory Animal Resource Center,State Key Lab for Animal Disease Control and Prevention,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin,Heilongjiang 150069)

机构地区：[1]中国农业科学院哈尔滨兽医研究所,动物疫病防控全国重点实验室,国家禽类实验动物资源库,黑龙江哈尔滨150069

出　　处：《中国家禽》2024年第11期70-77,共8页China Poultry

基　　金：“十四五”重点研发计划项目(2021YFD1800201)。

摘　　要：为了解H6N3亚型禽流感病毒(AIV)的遗传演化特点,试验对2023年采集自广东、湖北两地的活禽交易市场禽类咽喉拭子及泄殖腔拭子进行病原分离鉴定,并对分离的H6N3亚型AIV进行全基因组序列测定,进一步分析其遗传演化情况和特殊分子标记。结果显示:共分离到5株H6N3亚型AIV,且均为新型重配病毒,可分为2种基因型,其中4株湖北分离株属于基因1型,NA基因进化支相对独立,其余基因片段与分离于我国家禽的H3N2、H3N3、H3N8、H6N6亚型AIV有着较近的亲缘关系;1株广东分离株属于基因2型,与多种不同亚型野鸟源病毒高度同源;5株分离株HA蛋白的裂解位点序列仅有一个碱性氨基酸,符合低致病性禽流感病毒(LPAIV)的特征;4株湖北分离株的NA基因全长仅有1365bp,其编码的NA蛋白出现茎部缺失;部分分离株HA蛋白获得A138S突变,一株病毒的PB2蛋白获得I292V突变。综上所述,分离的5株H6N3亚型AIV为新型重配病毒,部分分离株获得哺乳动物适应性突变,应加强对H6亚型AIV的流行病学监测。To understand the genetic evolution characteristics of H6N3 subtype avian influenza virus(AIV),throat swabs and cloacal swabs were collected from live poultry markets in Guangdong and Hubei province in 2023 to isolate and identify the viruses.The whole genome of H6N3 subtype AIV were sequenced,and the genetic evolution and special molecular markers were analyzed.The results showed that 5 strains of H6N3 subtype AIV were isolated,which were novel reassortment viruses that could be divided into 2 genotypes.The 4 strains isolated from Hubei province belonged to genotype 1,the NA gene of which constituted a single branch,and the other gene segments were closely related to H3N2,H3N3,H3N8 and H6N6 subtypes AIV isolated from poultry in China.One strain isolated from Guangdong province belonged to genotype 2,and the genes of this virus were highly homologous with different subtypes AIV from wild birds.There was only one basic amino acid in the cleavage site of HA protein of 5 isolates,which were consistent with the low pathogenic avian influenza virus(LPAIV).The length of the NA gene of 4 strains isolated from Hubei province was only 1365 bp,with a deletion in the stalk of NA.The A138S substitutions was found in HA protein of some viruses,and the I292V was found in the PB2 of 1 isolate.In conclusion,the 5 strains of H6N3 subtype AIV were novel reassortment viruses and some strains had acquired mammalian adaptive substitutions,and epidemiological surveillance of H6 subtype AIV should be intensified.

关 键 词：H6N3 禽流感病毒 活禽交易市场 分子特征 基因重配 

分 类 号：S852.65[农业科学—基础兽医学]