高原鼢鼠和高原鼠兔CYP3A25基因的分子表征及功能分析  

Molecular characterization and functional analysis of CYP3A2 gene in plateau zokor and plateau pika

作　　者：谭宇尘 王春霖[2] 王静[2] 姚宝辉 师尚礼[1] 苏军虎[1] TAN Yu-chen;WANG Chun-lin;WANG Jing;YAO Bao-hui;SHI Shang-li;SU Jun-hu(College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Grassland Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou 730070,China;Lanzhou Vocational and Technical College,Lanzhou 730020,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]兰州职业技术学院,甘肃兰州730020

出　　处：《草原与草坪》2024年第5期43-50,共8页Grassland and Turf

基　　金：甘肃省高校产业支撑项目(2022CYZC-47);甘肃省重点研发专项(生态文明建设)(24YFFA058);甘肃省林草局科技支撑项目(LCJ2021020)。

摘　　要：【目的】探究高原鼢鼠(Eospalax baileyi)和高原鼠兔(Ochotona curzoniae)CYP3A25基因的分子结构及蛋白质的三维构象差异。【方法】检测高原鼢鼠和高原鼠兔CYP3A25基因外显子的核苷酸序列情况,并结合其他7种啮齿动物分析其氨基酸序列和蛋白质空间构象差异。【结果】在PCR结果检测中成功获得高原鼢鼠和高原鼠兔CYP3A25基因CDS区,长度均为1307 bp,并检测出基因序列差异及蛋白质空间构象差异。系统发育树构建发现高原鼢鼠与色列盲鼹鼠(Nannospalax galili)属于同一分支,高原鼠兔更趋同于智人(Homo sapiens)。对CYP3A25基因序列差异介导蛋白质空间构象改变分析后,发现高原鼢鼠和高原鼠兔相较小家鼠都有3个氨基酸序列差异导致的蛋白质空间构象改变。【结论】研究表明不同物种CYP3A25基因存在较大的序列差异,并且这种序列差异影响了蛋白质的空间构象。说明不同物种在CYP3A25基因的分子表征和潜在的解毒代谢能力上存在较大差异。研究为理解不同物种的解毒代谢基因差异提供了理论依据。【Objective】This study aimed toinvestigate the molecular structure and spatial conformation of the CYP3A25 gene in plateau zokor(Eospalax baileyi)and plateau pikas(Ochotona curzoniae).【Method】Nucleotide sequences of the CYP3A25 gene exons were detectedin both species and,and amino acid sequences and protein spatial conformations were analyzed in comparison with seven other rodent species.【Result】PCR successfully amplifiedthe CDS region of the 1307 bplong CYP3A25 genefor bothzokors and pikas,revealingdifferences in gene sequence and protein spatial conformation.A phylogenetic tree was constructed,demonstrating that the plateau zokor and the blind mole(Nannospalax galili)were closely related,while the plateau pika was more comparable to humans(Homo sapiens).Analysis of CYP3A25 gene variation revealedthree amino acid mutations in plateau zokor and plateau pika,resulting in changes to protein spatial conformation compared to Mus.【Conclusion】This study identifiessignificant sequence differences in the CYP3A25 gene among different species,impacting the spatial conformation of proteins.These findings suggestimportant variations in molecular characteristics and potential detoxification metabolism across species,providing insights into genetic differences in detoxification pathways.

关 键 词：高原鼢鼠 适应能力差异 CYP3A25基因 氨基酸序列 蛋白质空间构象 

分 类 号：S812.6[农业科学—草业科学]