高原鼢鼠精子发生相关基因的季节性表达特征  

Seasonal characteristics of spermatogenesis related gene expression in male plateau zokor

作　　者：姚宝辉 安康 侯齐琪[1,2] 张德罡 苏军虎[1,2,3] YAO Bao-hui;AN Kang;HOU Qi-qi;ZHANG De-gang;SU Jun-hu(College of Grassland Science,Key Laboratory of Grassland Ecosystem(Ministry of Education),Pratacultural Engineering Laboratory of Gansu Province,Sino‒U,S,Centers for Grazing land Ecosystem Sustainability,Gansu Agricultural University,Lanzhou 730070,China;Gansu Agricultural University-Massey University Research Centre for Grassland Biodiversity,Lanzhou 730070,China;Gansu Qilianshan Grassland Ecosystem Observation and Research Station,Tianzhu 733200,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃农业大学-新西兰梅西大学草地生物多样性研究中心,甘肃兰州730070 [3]甘肃省祁连山草原生态系统野外科学观测研究站,甘肃天祝733200

出　　处：《草原与草坪》2024年第5期61-71,共11页Grassland and Turf

基　　金：甘肃省科技计划项目(20JR10RA564);甘肃省教育厅产业支撑项目(2022CYZC-47);甘肃省林草局科技支撑项目(LCJ2021020);武威市市级科技计划项目(WW2002YFS008)。

摘　　要：【目的】探明高原鼢鼠(Eospalax baileyi)精子发生相关基因表达的季节性特征。【方法】分析高原鼢鼠繁殖期和非繁殖期睾丸形态、血清激素和睾丸特异性基因的季节特征。【结果】高原鼢鼠在繁殖期睾丸重量增加,睾酮分泌升高,初级精母细胞、次级精母细胞、单倍体精细胞和精子中特异性基因的表达水平均上调。非繁殖期睾丸萎缩,睾酮分泌水平降低,初级精母细胞、次级精母细胞、单倍体精细胞和精子中特异性基因的表达水平下调。【结论】高原鼢鼠睾丸形态、激素和基因表达具有季节性差异,非繁殖期高原鼢鼠睾丸精子发生停滞在精原细胞阶段。这为揭示高原鼢鼠睾丸发育机理提供了参考,对了解动物季节性繁殖的调控有重要意义。【Objective】Seasonal breeding animals exhibit annual periodic changes in gonadal morphology,physiological hormones levels and gene expression,which can improve reproductive efficiency and offspring survival rate.This study aims to explore the seasonal characteristics of spermatogenesisrelated gene expression in male plateau zokors.【Method】We analyzed testicular morphology,serum hormones levels and testiculisspecific genes between breeding season and nonbreeding season in plateau zokors.【Result】During the breeding season,male plateau zokor testes increased in size,testosterone levels were elevated,and a variety of germ cells were present in seminiferous tubles.The expression levels of specific genes were upregulated in primary spermatocytes,secondary spermatocytes,haploid spermatocytes and sperm.In contrast,during the nonbreeding season,the testes were small and the level of testosterone were insufficient.The expression levels of specific genes were downregulated in primary spermatocytes,secondary spermatocytes,haploid spermatocytes and sperm.【Conclusion】Our findings indicate seasonal differences in testicular morphology,hormone and gene expression of plateau zokor.Spermatogenesis arrest at the spermatogonia stage during the nonbreeding season.These results provide a valuable insight for into the mechanisms of testicular activity,and understanding the regulation of seasonal reproduction of animals.

关 键 词：季节性繁殖 睾酮 生殖细胞 基因表达 高原鼢鼠 

分 类 号：S812.6[农业科学—草业科学]