桃色欧文氏菌Cp2脂多糖对秀丽隐杆线虫生长发育的影响  

Effect of Cp2 lipopolysaccharides of Erwinia persicina on the growth and development of Caenorhabditis elegans

作　　者：张宏 黄荣 姚博 赵亮[1] 张振粉[1] ZHANG Hong;HUANG Rong;YAO Bo;ZHAO LIANG;ZHANG Zhen-fen(College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem,Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S.Center for Grassland Ecosystem Sustainability,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草原与草坪》2024年第5期82-87,98,共7页Grassland and Turf

基　　金：国家自然科学基金(32060369);甘肃省杰出青年基金(20JR10RA562);草业生态系统教育部重点实验室-人才培育项目(KLGE202203)。

摘　　要：【目的】探究桃色欧文氏菌Cp2脂多糖(Erwinia persicina Cp2 Lipopolysaccharides,Cp2-LPSe)对秀丽隐杆线虫(Caenorhabditis elegans)生长发育的影响。【方法】以野生型秀丽隐杆线虫N2为材料,用试剂盒法提取纯化Cp2-LPSe,用不同浓度Cp2-LPSe(0、0.157、0.314、0.471、0.628与0.785 EU/mL)处理秀丽隐杆线虫,测定其咽泵次数、繁殖能力、体脂含量、寿命长短、运动速度、运动振幅、身体弯曲频率等指标。【结果】各Cp2-LPSe浓度的处理下,秀丽隐杆线虫的生长发育总体呈现先上升后下降的趋势,当浓度低于0.314 EU/mL Cp2-LPSe时,除体脂含量外各指标均显著高于对照(P<0.05),并在0.314 EU/mL达到最大值;浓度超过0.314 EU/mL时,各指标均显著低于对照(P<0.05)。【结论】低于0.314 EU/mLCp2-LPSe处理秀丽隐杆线虫会提高其咽泵次数、运动速度、身体弯曲频率、运动振幅,在体脂含量不增高的前提下提高其繁殖能力、延长其寿命,促进其生长发育。本研究为Cp2-LPSe作为一种延缓机体衰老制剂的开发提供理论依据。【Objective】The study is aimed to investigate the effect of Cp2 Lipopolysaccharides(Cp2-LPSe)of Erwinia persicina on the growth and development of Caenorhabditis elegans.【Method】In this experiment,Cp2-LPSe was extracted and purified by kit method using wild-type Caenorhabditis elegans N2 as the material.Different concentrations of Cp2-LPSe(0,0.157,0.314,0.471,0.628 and 0.785 EU/mL)were used to treat Caenorhabditis elegans with different concentrations of Cp2-LPSe(0,0.157,0.314,0.471,0.628 and 0.785 EU/mL)to determine the number of throat pumps,reproductive capacity,body fat content,life span,movement speed,movement amplitude,body bending frequency and other indicators.【Result】The experiment showed that the growth and development of Caenorhabditis elegans had a general trend of first rising and then decreasing under the treatment of each Cp2-LPSe concentration.When the concentration of Cp2-LPSe was 0.314 EU/mL,all indexes except body fat content reached a maximum and were significantly higher than those of the control(P<0.05),and when the concentration exceeded 0.314 EU/mL,all indexes were significantly lower than those of control(P<0.05).【Conclusion】0.314 EU/mL Cp2-LPSe treatment of Caenorhabditis elegans could increase the number of throat pumps,movement speed,body bending frequency and movement amplitude,improve its reproductive ability,prolong its lifespan,and promote its growth and development without increasing its body fat content.This study provides a theoretical basis and practical basis for the development of Cp2-LPSe as a anti-aging agent.

关 键 词：桃色欧文氏菌脂多糖 秀丽隐杆线虫 生长发育 

分 类 号：Q936[生物学—微生物学]