盐胁迫下植物根际促生菌对垂穗披碱草生长及生理生化的影响  

Effects of plant growth-promoting rhizobacteria on growth,physiological,and biochemical responses to salt-stressed Elymus nutans

作　　者：李青璞 白建海 姚拓[1] 李明源 王振龙 张洁 李智燕 LI Qing-Pu;BAI Jian-Hai;YAO Tuo;LI Ming-Yuan;WANG Zhen-Long;ZHANG Jie;LI Zhi-Yan(College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Grassland Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land cosystem Sustainability,Lanzhou 730070,China;Gansu Xinhai Herbage Feed Technology Co,Lanzhou,730070,China;Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region,Kashi,844006,China;Key Laboratory of Grassland Ecosystem Grassland Technical Extension Station of Gansu Province,Lanzhou,730070,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃欣海牧草饲料科技有限公司,甘肃兰州730070 [3]新疆帕米尔高原生物资源与生态自治区重点实验室,新疆喀什844006 [4]甘肃省草原技术推广总站,甘肃兰州730070

出　　处：《草原与草坪》2024年第5期115-123,共9页Grassland and Turf

基　　金：甘肃省林草局科技创新项目(KJCX2021010);高寒退化草地植被恢复与土壤改良菌剂研发示范项目(GSAU-TSYF-2021-011)。

摘　　要：【目的】探究植物根际促生菌对垂穗披碱草在盐渍化土壤中生长的影响。【方法】以课题组前期分离的4株植物根际促生菌(PGPR)为试验材料,设置不同盐浓度处理,采用沙培法种植垂穗披碱草(Elymus nutans),将4株植物根际促生菌[(TznFn7、MYnI4、MYP(m)Jn8和TznG2)]接种剂接种在种植垂穗披碱草的河沙中,测定不同菌株处理和不同盐浓度下垂穗披碱草生长指标和生理特性,研究垂穗披碱草在菌株接种剂处理下的耐盐性。【结果】在150 mmol/L盐浓度下,与CK相比各接种处理的CAT活性提高2.08%~7.35%,接种菌株TznFn7、TznG2处理下与CK相比POD活性提高了27.24%、13.30%,各接种处理下SOD活性提高5.18%~24.03%,与CK相比各接种处理的MDA含量降低25.87%~47.53%。【结论】在盐胁迫下,接种不同PGPR菌剂不仅促进垂穗披碱草生长,同时有效提高了植物CAT、POD和SOD抗氧化酶活性,降低了植物细胞MDA含量。本研究选用的PGPR菌株改善了盐胁迫下植物生长,减轻了植物细胞损伤,提高了植物耐盐性,为盐渍化土壤的利用提供了一定的理论和实践基础。【Objective】This study investigated the effects of plant growthpromoting rhizobacteria(PGPR)on the growth of Elymus nutanssalinized soil.【Method】Four plant PGPR strains isolated by our research group(TznFn7,MYnI4,MYP(m)Jn8 and TznG2)were tested under varying salt concentrations.E.nutanswas cultivated in a sand culture system,andthe growth indices and physiological characteristics were assessed under different strain treatments and saltlevels.The study focused on salt tolerance of E.nutans following PGPR innoculation.【Result】Results demonstrated that at a salt concentration of 150 mmol/L,catalase(CAT)activity increased by 2.08%to 7.35%,while peroxidase(POD)activity rose by 27.24%and 13.30%with TznFn7 and TznG2 inoculations,compared to control(CK).Superoxide dismutase(SOD)activity increased between 5.18%and 24.03%across all innoculatedstrains,and malondialdehyde(MDA)content decreased from 25.87%to 47.53%compared to CK.Inoculation with different PGPR strains not only promoted the growth of E.nutans under salt stress but also enhanced the activities of CAT,POD and SOD,while reducing MDA content in plant cells.This alleviated the cellular damage and improved the plantsresilience to saline conditions.【Conclusion】The PGPR strains selected in this study significantly enhanced plant growth and salt tolerance,providing a theoretical and practical basis for the utilization of saline soils.

关 键 词：盐胁迫 植物根际促生菌 垂穗披碱草 生理 响应 

分 类 号：S543[农业科学—作物学]