基于低共熔溶剂的牛皮胶原提取工艺研究  

Extraction of Collagen from Cowhide by Deep Eutectic Solvent Aqueous Solutions

作　　者：俞小凤 宋文祥 许波 刘颖 王平 YU Xiaofeng;SONG Wenxiang;XU Bo;LIU Ying;WANG Ping(College of Textile Science and Engineering,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学纺织科学与工程学院,江苏无锡214122

出　　处：《皮革科学与工程》2024年第6期24-30,共7页Leather Science and Engineering

基　　金：国家自然科学基金青年基金项目(22308122)。

摘　　要：以脱灰牛皮边角料为原料,使用低共熔溶剂(DES)对其中的胶原蛋白进行提取,研究了DES种类、浓度、提取时间和温度条件对提取效果的影响。结果表明,当使用摩尔比为1:5的尿素与乙酸组成的DES水溶液作为提取溶液,温度15℃,DES浓度0.75mol/L,时间24h,提取效果最好,胶原的提取率为37%;红外光谱结果显示,产物具有典型的I型胶原蛋白吸收峰。与传统酸法与酶法提取的胶原蛋白相比,使用DES提取的胶原蛋白纯度高,三股螺旋结构保留更好,在生物医用领域有应用前景。Deep eutectic solvent(DES)aqueous solutions were utilized to extract collagen from deliming cowhide trimmings.The effect of DES type,DES concentration,extraction time and temperature on the collagen extraction efficiency were investigated.Results showed that the optimal extraction efficiency can be obtained by using the DES containing urea and acetic acid as the extraction agent,with the DES concentration of 0.75 mol/L,temperature of 15°℃and extraction time of 24 h,respectively.Under this condition,the yield of collagen was 37%;the results of infrared spectroscopy showed that the product held a typical absorption peak of type I collagen.Compared with the collagen extracted by traditional acidic and enzymatic methods,the collagen extracted using DES aqueous solution displayed higher purity of collagen and intact triple helix structure,which endow it with wide potential in biomedical field.

关 键 词：低共熔溶剂 胶原 提取工艺 牛皮 

分 类 号：TS512[轻工技术与工程—皮革化学与工程]