黄芪多糖调控PI3K/AKT通路改善糖尿病大鼠肾损伤  

Astragalus polysaccharides improve renal injury in diabetic rats by regulating PI3K/AKT pathway

作　　者：吴东 张庆红[1] 何立群 杨山珊 WU Dong;ZHANG Qinghong;HE Liqun;YANG Shanshan(Department of Nephrology,Taihe Hospital Affiliated Hospital of Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院附属太和医院肾病内科,湖北十堰442000

出　　处：《生物技术》2024年第5期613-618,542,共7页Biotechnology

摘　　要：[目的]探究黄芪多糖对糖尿病大鼠肾损伤的作用与机制。[方法]把大鼠随机分为:对照组、模型组、黄芪多糖组、抑制剂组。黄芪多糖组的大鼠每天给与400 mg/kg黄芪多糖灌胃处理,抑制剂组的大鼠每天给与0.175 mg/kg的Wortmannin处理,持续8w。通过HE染色和PAS染色分析肾脏病理结构和糖原含量;通过检查血清BUN、SCr、TC和TG水平分析肾功能状态;通过ELISA试验检查血清炎症因子水平;通过TUNEL染色分析肾小球细胞凋亡率;通过蛋白免疫印迹试验分析肾脏组织PI3K/AKT通路蛋白表达水平。[结果]与糖尿病肾病组的大鼠比较,黄芪多糖和Wortmannin处理后,大鼠肾组织损伤和肾功能得到改善(13.36±0.57 vs 10.37±0.25 vs 9.28±0.77;72.01±2.39 vs 61.13±9.19 vs 56.28±6.29;2.58±0.27 vs 1.97±0.15 vs 1.88±0.27;0.96±0.17 vs 0.78±0.09 vs 0.71±0.11;P<0.05),肾小球细胞凋亡率和血清炎症因子水平降低(588.36±11.67 vs 502.17±9.25 vs 476.88±8.77;239.21±12.79 vs 182.33±7.29 vs 167.38±7.21;151.88±13.27 vs 112.17±6.15 vs 106.18±9.37;P<0.05),PI3K表达和AKT磷酸化水平降低。[结论]黄芪多糖能够降低糖尿病肾病大鼠血清炎症因子的含量(588.36±11.67 vs 502.17±9.25;239.21±12.79 vs 182.33±7.29;151.88±13.27 vs 112.17±6.15;P<0.05)、减少肾小球细胞凋亡(52.17%±9.25%vs 38.25%±2.19%;P<0.05)并恢复大鼠肾功能,改善STZ导致的肾组织损伤,并且这一作用与抑制PI3K/AKT信号通路相关(0.99±0.07 vs 0.51±0.03;0.79±0.08 vs 0.49±0.08;P<0.05)。[Objective]To investigate the effect of Astragalus polysaccharide(APS)on renal injury in diabetic rats and its related mechanism.[Method]Rats were randomly divided into 4 groups:control group,model group,APS group and inhibitor group.Rats in APS group were treated with 400 mg/kg APS daily by gavage,and rats in inhibitor group were treated with 0.175 mg/kg Wortmannin daily for 8 weeks.Renal pathological structure and glycogen content were analyzed by HE staining and PAS staining.Renal function was analyzed by detecting the levels of serum BUN,SCr,TC and TG.The levels of serum inflammatory factors were detected by ELISA test.The apoptosis rate of glomerular cells was analyzed by TUNEL staining.The protein expression levels of PI3K/AKT pathway in kidney tissues were analyzed by Western Blot.[Result]Compared with the diabetic nephropathy group,the renal tissue damage and renal function of the rats treated with AP and Wortmannin were improved(13.36±0.57 vs 10.37±0.25 vs 9.28±0.77;72.01±2.39 vs 61.13±9.19 vs 56.28±6.29;2.58±0.27 vs 1.97±0.15 vs 1.88±0.27;0.96±0.17 vs 0.78±0.09 vs 0.71±0.11;P<0.05),glomerular cell apoptosis rate and serum inflammatory cytokines levels were significantly decreased(588.36±11.67 vs 502.17±9.25 vs 476.88±8.77;239.21±12.79 vs 182.33±7.29 vs 167.38±7.21;151.88±13.27 vs 112.17±6.15 vs 106.18±9.37;P<0.05),and the expression of PI3K and AKT phosphorylation decreased.[Conclusion]Astragalus polysaccharides can reduce the content of serum inflammatory factors in diabetic nephropathy rats(588.36±11.67 vs 502.17±9.25;239.21±12.79 vs 182.33±7.29;151.88±13.27 vs 112.17±6.15;P<0.05),reduced glomerular cell apoptosis(52.17%±9.25%vs 38.25%±2.19%;P<0.05)and improved renal function and renal tissue injury induced by STZ,and this effect was related to the inhibition of PI3K/AKT signaling pathway(0.99±0.07 vs 0.51±0.03;0.79±0.08 vs 0.49±0.08;P<0.05).

关 键 词：黄芪多糖 磷脂酰肌醇-3激酶 蛋白激酶B 糖尿病肾病 凋亡 炎症 肾功能 糖原 

分 类 号：R285.5[医药卫生—中药学]