SHP-1调控NF-κB影响深静脉血栓血清炎症因子水平  

SHP-1 regulates NF-κB to affect the levels of inflammatory factors in deep vein thrombosis

作　　者：胡同山 胡玉菲 赵瑜潇 HU Tongshan;HU Yufei;ZHAO Yuxiao(Department of Clinical Laboratory,Xingtai Medical College(The First Hospital of Xingtai),Xingtai 054000,China)

机构地区：[1]邢台医学高等专科学校第一附属医院(邢台市第一医院)检验科,河北邢台054000

出　　处：《生物技术》2024年第5期624-628,共5页Biotechnology

基　　金：邢台市重点研发计划项目(2020ZC175)。

摘　　要：[目的]探究SHP-1与NF-κB对深静脉血栓血清炎症因子水平的影响。[方法]通过免疫组化和蛋白免疫印迹实验分析下肢静脉血管组织中的SHP-1表达水平;将深静脉血栓患者的外周血单个核细胞分为3组:对照组,pcDNA NC组和pcDNA SHP-1组。通过酶联免疫吸附试验分析外周血单个核细胞分泌的IL-1β、IL-6、IFN-γ和TNF-α的浓度;通过蛋白免疫印迹方法检测不同实验组的外周血单个核细胞中SHP-1以及NF-κB p65的蛋白水平。[结果]免疫组化实验结果显示,SHP-1在深静脉血栓患者的血管组织中表达降低(P<0.05),NF-κB p65在深静脉血栓患者的血管组织中血管组织中表达增加(0.21±0.01 vs 0.82±0.05,P<0.05);酶联免疫吸附试验结果显示,pcDNA SHP-1组的炎症因子IL-1β、IL-6、IFN-γ和TNF-α的释放水平下降(P<0.05);蛋白免疫印迹实验结果显示,pcDNA SHP-1组的SHP-1蛋白表达水平增加(0.19±0.02 vs 0.22±0.03 vs 0.82±0.06,P<0.05),而NF-κB p65蛋白表达水平降低(0.71±0.03 vs 0.73±0.05 vs 0.25±0.02,P<0.05)。[结论]在深静脉血栓患者体内,SHP-1表达下降能够导致NF-κB p65蛋白表达增加,进而促进外周血单个核细胞释放IL-1β、IL-6、IFN-γ和TNF-α等炎症因子,加重炎症反应程度,SHP-1是深静脉血栓治疗的潜在靶点。[Objective]To investigate the effect of SHP-1 and NF-κB on serum inflammatory factors in patients with deep vein thrombosis.[Method]Immunohistochemistry and Western Blot were used to analyze the expression level of SHP-1 in the vascular tissue of lower extremity veins.Peripheral blood mononuclear cells from patients with DVT were divided into three groups:control group,pcDNA NC group and pcDNA SHP-1 group.The concentrations of IL-1β,IL-6,IFN-γand TNF-αsecreted by peripheral blood mononuclear cells were analyzed by enzyme-linked immunosorbent assay.The protein levels of SHP-1 and NF-κB p65 in peripheral blood mononuclear cells of different experimental groups were detected by Western Blot.[Result]The results of immunohistochemistry showed that the expression of SHP-1 was decreased(P<0.05),and the expression of NF-κB p65 was increased(0.21±0.01 vs 0.82±0.05;P<0.05)in the vascular tissues of patients with deep vein thrombosis.The results of ELISA showed that the release levels of inflammatory factors IL-1β,IL-6,IFN-γand TNF-αin the pcDNA SHP-1 group were decreased(P<0.05).The results of Western Blot showed that the expression level of SHP-1 protein in the pcDNA SHP-1 group was increased(0.19±0.02 vs 0.22±0.03 vs 0.82±0.06;P<0.05),while the expression level of NF-κB p65 protein was decreased(0.71±0.03 vs 0.73±0.05 vs 0.25±0.02;P<0.05).[Conclusion]In patients with DVT,the decreased expression of SHP-1 can lead to the increased expression of NF-κB p65 protein,and then promote the release of inflammatory factors such as IL-1β,IL-6,IFN-γand TNF-αfrom peripheral blood mononuclear cells,and aggravate the degree of inflammatory response.SHP-1 was a potential target for the treatment of DVT.

关 键 词：SHP1 NF-ΚB 深静脉血栓 炎症 血清 免疫组化 外周血 骨折 

分 类 号：R543.6[医药卫生—心血管疾病]