过表达平邑甜茶MhbHLH122增强苹果愈伤组织耐盐性  

Overexpression of Malus hupehensis var.pingyiensis MhbHLH122 enhances salt tolerance in apple calli

作　　者：朱晓悦 宋建飞 程玉瑾 孙宝箴 张晓健 张玮玮 杨洪强[1] ZHU Xiaoyue;SONG Jianfei;CHENG Yujin;SUN Baozhen;ZHANG Xiaojian;ZHANG Weiwei;YANG Hongqiang(College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018,China)

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018

出　　处：《植物生理学报》2024年第10期1561-1568,共8页Plant Physiology Journal

基　　金：国家自然科学基金(32172517);国家重点研发计划(2019-YFD100103)。

摘　　要：本研究以苹果砧木平邑甜茶(Malus hupehensis var.pingyiensis)cDNA为模板,克隆出bHLH家族成员MhbHLH122;该基因编码区全长1314 bp,编码蛋白具有bHLH保守结构域,与桃PpbHLH122转录因子亲缘关系最近。将MhbHLH122转入‘王林’苹果愈伤组织,获得了MhbHLH122过表达的愈伤株系;在含200 mmol·L^(–1)NaCl的培养基上,过表达MhbHLH122的愈伤组织比野生型愈伤组织生长状态更好,其丙二醛(MDA)含量、细胞相对死亡率、过氧化氢(H_(2)O_(2))含量、超氧阴离子(O_(2)^(¯·))产生速率均低于野生型,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性均高于野生型,显示过表达MhbHLH122能增强苹果愈伤组织耐盐性。This paper used the cDNA of apple rootstock Malus hupehensis var.pingyiensis as a template,cloned MhbHLH122,a member of bHLH family which has a total length of 1314 bp gene coding region,its encoded protein contains a bHLH conserved domain and shares the highest similarity with the peach PpbHLH122 transcription factor.Transferring MhbHLH122 into calli of‘Orin’apple resulted in the overexpression of MhbHLH122 in calli.On a culture medium containing 200 mmol·L^(–1)NaCl,the growth status of calli overexpressing MhbHLH122 was better than that of the wild-type,the malondialdehyde(MDA)content,relative cell death rate,hydrogen peroxide(H_(2)O_(2))content,and superoxide anion(O_(2)^(¯·))production rate were all lower than in the wild-type,the activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)were all higher than the wild-type,in the overexpressed MhbHLH122 compared to the wildtype,suggested that the overexpression of MhbHLH122 can improve salt tolerance in apple calli.

关 键 词：MhbHLH122 转录因子 苹果愈伤组织 盐胁迫 

分 类 号：S661.1[农业科学—果树学]