3种植物源化合物对杂拟谷盗的毒力及几种酶活性的影响  

Toxicity of Three Plant Compounds and Effect of Enzymes on Tribolium confusum

作　　者：石丹妮 康鹏 常静[1] 马崇勇 嘎丽娃 赵万凯 温雅欣 李海平[1] Shi Danni;Kang Peng;Chang Jing;Ma Chongyong;Ga Liwa;Zhao Wankai;Wen Yaxin;Li Haiping(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Huhehaote 010019;Forestry and Grassland Pest Control and Quarantine Station of Inner Mongolia Autonomous Region,Huhehaote 010020)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,呼和浩特010019 [2]内蒙古自治区林业和草原有害生物防治检疫总站,呼和浩特010020

出　　处：《中国粮油学报》2024年第10期11-19,共9页Journal of the Chinese Cereals and Oils Association

基　　金：草原瑞香狼毒发生、防控与利用研究及地方标准制定项目(20210621)。

摘　　要：杂拟谷盗(Tribolium confusum)是一种重要的储粮害虫,为明确3种植物源化合物新狼毒素A、百里香酚和印楝素对杂拟谷盗的毒杀作用,研究了3种植物源化合物对杂拟谷盗的熏蒸和拒食作用,以及对杂拟谷盗2种靶标酶离体活性的抑制和对3种解毒酶活性的影响。结果显示,新狼毒素A、百里香酚和印楝素熏蒸杂拟谷盗24 h后,致死中浓度LC 50分别为28.366、51.009、42.201 mg/L;非选择性拒食处理杂拟谷盗5 d后的拒食中浓度AFC 50分别为6.630、2.862和0.489 mg/L。3种化合物对杂拟谷盗离体AChE和ATPase活性均有抑制作用。3种化合物处理杂拟谷盗后,细胞色素P450和GST活性被诱导升高;而CarE活性降低,表现为抑制作用。3种植物源化合物对杂拟谷盗具有一定的毒杀作用,对AChE、ATPase和CarE有抑制作用,对细胞色素P450和GST活性有诱导作用。Tribolium confusum is an important grain stored pest.The toxicity and mechanism of Neochamaejasmine A,thymol and azadirachtin against T.confusum were tested.The fumigation and antifeedant effect of three plant compounds against T.confusum were determined,and the inhibition of three plant compounds to two target enzymes activities in vitro and the effects on three detoxification enzymes activities were measured in this paper.The results indicated that the LC 50 of Neochamaejasmine A,thymol and azadirachtin to T.confusum were 28.366,51.009 and 42.201 mg/L after 24 h of fumigation,and AFC 50 of non-selective antifeedant treatment were 6.630,2.862 and 0.489 mg/L after 5 d,respectively.The activities of AChE and ATPase could be inhibited by three plant compounds in vitro.After treatment with three plant compounds to T.confusum,the activities of cytochrome P450 and GSTs were induced to increase,and the activity of CarE was inhibited.In summary,the three plant compounds have certain toxic effects on T.confusum,and activities of AChE,ATPase and CarE were inhibited,and the activities of cytochrome P450 and GST swere induced.

关 键 词：杂拟谷盗 植物源化合物 毒力 靶标酶 解毒酶 

分 类 号：S481.1[农业科学—农药学]