棉袜中微生物菌落结构分析及分离鉴定  

Analysis,Isolation and Identification of Microbial Colony Structure in Cotton Socks

作　　者：王梦娣 任泽华 王婷霞 方冠宇 刘颖昕 杨青波 刘建立 WANG Mengdi;REN Zehua;WANG Tingxia;FANG Guanyu;LIU Yingxin;YANG Qingbo;LIU Jiani(College of Textile Science and Engineering,Jiangnan University,Wuxi 214122,China;College of Food and Health,Zhejiang A&F University,Hangzhou 311300,China;Wuxi Little Swan Electrical Appliance Co.,Ltd.,Wuxi 214035,China)

机构地区：[1]江南大学纺织科学与工程学院,江苏无锡214122 [2]浙江农林大学食品与健康学院,浙江杭州311300 [3]无锡小天鹅电器有限公司,江苏无锡214035

出　　处：《服装学报》2024年第5期377-383,共7页Journal of Clothing Research

基　　金：江苏省研究生科研与实践创新计划项目(SJCX22_1109)。

摘　　要：为了探究居民穿过的棉袜表面微生物的种类和菌群结构,以广东地区为例,通过高通量测序技术分析棉袜表面的真菌群落组成,并结合培养基划线分离纯化对棉袜表面的优势真菌进行鉴定。从棉袜表面初步分离鉴定出5种不同的真菌,并对真菌核糖体DNA内部转录间隔区序列进行分析,确定其为烟曲霉、黑曲霉、短柄帚霉、杂色曲霉和球孢枝孢。采用高通量测序技术分析真菌群落组成,发现棉袜中占绝对优势的真菌门为子囊菌门和担子菌门,平均相对丰度大于5%的真菌属为枝孢菌属、红酵母属、假丝酵母属和曲霉菌属。采用FUNGuild进行功能预测,发现棉袜中真菌群落的功能以腐生为主。研究结果为抑菌袜的研发和真菌交叉感染的预防提供参考。In order to explore the diversity and structure of microorganisms on the surface of cotton socks worn by residents,taking Guangdong Province as an example,the fungal community composition on the surface of cotton socks was analyzed by high-throughput sequencing technology,and the dominant fungi on the surface of cotton socks were identified with the isolation and purification of culture medium.Five different fungi were isolated and identified from the surface of cotton socks by 18S rDNAITS sequence analysis.They were identified as Aspergillus fumigatus,Aspergillus niger,Scopulariopsis brevicaulis,Aspergillus variegatus and Cladosporium sphaerosporum.Through the analysis of fungal community composition by high-throughput sequencing technology,it was found that Ascomycetes and Basidiomycetes were the predominant fungal phyla in cotton socks,and Cladosporium,Rhodotorula,Candida and Aspergillus were the fungal genera with average abundance greater than 5%.FunGuild function prediction shows that the function of fungal community in cotton socks is mainly saprophytic.The results provide reference for the research and development of antibacterial socks and the prevention of fungal cross infection.

关 键 词：棉袜 高通量测序 菌落结构 纯化与鉴定 序列分析 

分 类 号：TS116[轻工技术与工程—纺织材料与纺织品设计] S435.62[轻工技术与工程—纺织科学与工程]