三种病原体核酸检测用假病毒标准品的制备和评价  

Preparation and Evaluation of Pseudovirus Standards for Ucleic Acid Detection of Three Pathogens

作　　者：林婧 范宇宸 侯春燕 王齐鑫 雍雪青 LIN Jing;FAN Yuchen;HOU Chunyan;WANG Qixin;YONG Xueqing

机构地区：[1]南京市计量监督检测院

出　　处：《计量与测试技术》2024年第10期101-105,共5页Metrology & Measurement Technique

基　　金：南京市市场监督管理局科技项目(项目名称:新型冠状病毒假病毒抗原标准品的制备及标准评价体系的建立,项目编号:Kj2022025);江苏省药品监督管理局科技项目(项目名称:病毒抗原标准品的研制及药物抗病毒活性标准评价体系的建立,项目编号:202359)。

摘　　要：本文通过制备和定值的人类免疫缺陷病毒、狂犬病毒、新型冠状病毒的标准品,使用内部含有特异性核酸序列的慢病毒体系进行假病毒包装和基因测序,并采用数字PCR方法定值,对其均匀性、稳定性和适用性进行评价,从而确定特征量值及扩展不确定度(k=2)。结果表明:3种假病毒核酸序列与NCBI数据库中的序列一致。In this paper,the standard products of human immunodeficiency virus,rabies virus and novel coronavir-us were prepared and valued,and the lentivirus system containing specific nucleic acid sequence was used for pseudovirus packaging and gene sequencing,and the uniformity,stability and applicability of the lentivirus were e-valuated by digital PCR method,so as to determine the eigenvalue and extended uncertainty(k=2).The results showed that the nucleic acid sequences of the three pseudoviruses were consistent with those in the NCBI database.

关 键 词：新型冠状病毒 人类免疫缺陷病毒 狂犬病毒 数字PCR 假病毒标准品 

分 类 号：S85[农业科学—兽医学]