虫草素通过激活Nrf2/HO-1/NQO1通路缓解脑缺血再灌注大鼠脑组织损伤  

Cordycepin Relieves Brain Tissue Damage in Rats with Cerebral Ischemia and Reperfusion by Activating Nrf2/HO-1/NQO1 Pathway

作　　者：吴双[1] 代绪波[1] 崔珍 姜风英 庞素秋 WU Shuang;DAI Xubo;CUI Zhen;JIANG Fengying;PANG Suqiu(Department of Clinical Laboratory,Joint Logistics Support Force 910th Hospital,Quanzhou,Fujian,362000,China;Department of Clinical Laboratory,Beijing Jishuitan Hospital,Beijing,100035,China;Department of Clinical Laboratory,Suzhou University Affiliated Wuxi Ninth Hospital,Wuxi,Jiangsu,214063,China;Disease Control and Prevontion,Joint Logistics Support Force 910th Hospital,Quanzhou,Fujian,362000,China)

机构地区：[1]中国人民解放军联勤保障部队第910医院检验科,福建省泉州市362000 [2]北京积水潭医院检验科,北京市100035 [3]苏州大学附属无锡九院检验科,江苏省无锡市214063 [4]联勤保障部队第910医院疾病预防控制科,福建省泉州市362000

出　　处：《医学分子生物学杂志》2024年第6期530-536,共7页Journal of Medical Molecular Biology

基　　金：福建省泉州市科技项目(No.2018N136S);国家自然科学基金促进海峡两岸科技合作联合基金(No.U1405215)。

摘　　要：目的探讨虫草素(cordycepin,COR)在脑缺血再灌注(cerebral ischemia reperfusion,CI/R)大鼠脑组织损伤中的保护作用及机制。方法60只Wistar大鼠分为健康对照组、模型组、模型加药组。跳台试验和Y迷宫实验检测大鼠学习和记忆力;HE染色法检测脑组织病理变化;干湿重法计算脑含水率和脑指数;TUNEL法观测脑组织细胞凋亡情况;ELISA测定大鼠血清中超氧化物歧化酶(superoxide dismutase。SOD)、丙二醛(malonic dialdehyde,MDA)、乳酸脱氢酶(lactate dehydrogenase,LDH)的含量;蛋白质印迹检测Bax/Bcl-2、cleaved cas9/cas9、cleaved cas3/cas3、Nrf2、HO-1和NQO1蛋白表达水平。结果与模型组比较,COR能剂量依赖地改善大脑组织损伤,抑制氧化应激,促进Nrf2/HO-1/NQO1通路激活。结论虫草素能够改善脑组织损伤,其作用机制与上调Nrf2通路有关。Objective To investigate the protective effect of cordycepin(COR)on brain tissue damage in rats with cerebral ischemia-reperfusion,and to preliminarily clarify the protective mechanism.Methods Sixty Wistar rats were divided into 3 groups:Normal control group,model group,and model plus drug group.Detection of learning and memory capacities in rats by step-down test and Y maze test.HE staining was employed to observe the pathological changes of brain tissues.Dry and wet weight ratio was used to calculate brain water content and brain index.TUNEL was used to observe cell apoptosis in brain tissues.The levels of serum SOD,MDA and LDH of rats were determined by ELISA.Western blotting was used to detect the expression levels of Bax/Bcl-2,cleaved cas9/cas9,cleaved cas3/cas3,Nrf2,HO-1 and NQO1 proteins.Results Compared with the model group,COR can dose dependently improve brain tissue damage,inhibit oxidative stress,and promote activation of the Nrf2/HO-1/NQO1 pathway.Conclusion Cordycepin can improve brain tissue damage by upregulating the Nrf2 pathway.

关 键 词：虫草素 脑缺血再灌注 凋亡 超氧化物歧化酶 Nrf2/HO-1/NQO1通路 

分 类 号：R965.1[医药卫生—药理学]