双咪唑硝基衍生物银(Ⅰ)配合物的合成及抗厌氧菌活性  

Synthesises and anti-anaerobic activities of silver (Ⅰ) complexes involving diimidazole nitro-derivative

作　　者：杨莉宁[1] 范涛[1] 姜箴一 姚琳[1] 胡娅琪 王荣[1] 余丽丽[1] YANG Lining;FAN Tao;JIANG Zhenyi;YAO Lin;HU Yaqi;WANG Rong;YU Lili(School of Pharmacy,Medical University of Xi′an,Xi′an,Shaanxi 710021,China)

机构地区：[1]西安医学院药学院,陕西西安710021

出　　处：《贵州师范大学学报（自然科学版）》2024年第6期35-43,66,共10页Journal of Guizhou Normal University：Natural Sciences

基　　金：国家自然科学基金资助项目(81903288);陕西省高校青年创新团队建设项目(2022-85);陕西省科技厅自然科学基金项目(2022JM-492);陕西省教育厅服务地方专项项目(产业化培育项目)(23JC060);西安医学院校级科技创新团队项目(2021TD07);西安医学院2022年度科研能力提升计划项目(2022NLTS081);陕西省大学生创新创业训练项目(S202311840037)。

摘　　要：以柔性双咪唑硝基衍生物1,3-二[1-(2-甲基-4-硝基咪唑)]丙烷(L1)和1,4-二[1-(2-甲基-4-硝基咪唑)丁烷(L2)分别与硝酸银发生反应,得到配合物{[Ag(L1)NO_(3)]·H_(2)O}_n(1)和[Ag(L2)_(2)NO_(3)]·2H_(2)O(2)。采用元素分析、摩尔电导率、紫外光谱、红外光谱、固体荧光光谱、电喷雾质谱及热重分析等方法对配合物(1)、配合物(2)的组成和化学结构进行了表征与确认。通过光谱法研究了配合物与小牛胸腺DNA(ct-DNA)的相互作用,并测试了配合物对ct-DNA黏度的影响。结果表明配合物(1)、配合物(2)分别以插入、部分插入模式与DNA作用。抗厌氧菌活性实验发现,配合物(1)对厌氧菌变形链球菌(Streptococcus mutans,UA 159)和伴放线放线杆菌(Aggregatibacter actinomycetemcomitans,ATCC 29523)的抗菌活性较强,最小抑菌浓度MIC为20~40μg/mL,推测银(Ⅰ)配合物的抗菌机理有可能部分靶向细菌的DNA。固体荧光光谱实验表明配合物(1)的荧光发射光谱强度远远高于其有机配体,几乎达到其有机配体发射强度的20倍,在光致发光领域具有潜在的应用前景。Two silver(Ⅰ)complexes{[Ag(L1)NO_(3)]·H_2O}_n(1),[Ag(L2)_2NO_(3)]·2H_2O(2)have been synthesized from diimidazole nitro-derivative 1,3-bis[1-(2-methyl-4-nitro imidazole)] propane(L1),1,4-bis[1-(2-methyl-4-nitroimida zole)butane(L2)and silver nitrate,respectively.Compositions and chemical structures of the complexes were characterized by elemental analysis,Uv-vis,FT-IR,solid fluorescence spectra,TG-DSC,ESI-MS and conductivity analysis.The binding properties of the complexes toward calf thymus DNA(ct-DNA)were figured out via electronic absorption,fluorescence spectra.Viscosity measurements were carried out to clarify further the interaction mode of compounds with DNA.Experimental results indicate that complex(1),complex(2)bind to DNA by intercalation,partial intercalation mode specifically.The antibacterial activities test show that complex {[Ag(L1)NO_(3)]·H_2O}_n(1)displayed favorable activity of anti-anaerobic bacteria against Streptococcus mutans(UA 159)and Aggregatibacter actinomycetemcomitans(ATCC 29523)with MIC values of 20~40 μg/mL,suggesting that the antibacterial mechanism of silver(I)complex may partially target the DNA of bacteria.At the same time,{[Ag(L1)NO_(3)]·H_2O}_n(1)exhibite much stronger fluorescence emission than its ligand,and is expected to be used in the field of photoluminescence.

关 键 词：双咪唑硝基衍生物 银(Ⅰ)配合物 与DNA相互作用 抗厌氧菌活性 荧光性质 

分 类 号：O614[理学—无机化学]