Survey and Detection of Disease and Pest Occurrence in Citrus Nurseries  

作　　者：Liying GUO Qinghao SONG Ying HE Jiayi TANG Xiaoting ZHUANG Guochan HUANG Qianhua JI Yaping HU 

机构地区：[1]Institute of Pomology,Zhaoqing University,Zhaoqing 526361,China [2]College of Life Sciences,Zhaoqing University,Zhaoqing 526361,China [3]Sihui Science and Technology Demonstration Park,Zhaoqing 526061,China

出　　处：《Plant Diseases and Pests》2024年第5期19-23,共5页植物病虫害研究（英文）

基　　金：Supported by Earmarked Fund for China Agriculture Research System(CARS-26);Science and Technology Innovation Guidance Project of Zhaoqing City(2023040308008);Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Province(S202310580050);Project of High-quality Development in Hundred Counties,Thousands Towns and Ten Thousand Villages.

摘　　要：[Objectives]The paper was to ascertain the prevalence of diseases and pests in a range of citrus nurseries situated in Guangdong Province and its neighboring provinces.[Methods]Citrus diseases and pests were systematically investigated,and citrus leaf samples were randomly collected from 15 citrus nurseries across 8 regions in Guangdong Province and its neighboring provinces.Quantitative polymerase chain reaction(qPCR)and reverse transcription polymerase chain reaction(RT-PCR)techniques were employed to detect diseases in the collected samples.Additionally,root and substrate samples were obtained,and root-knot nematodes were isolated using the Baermann funnel method.[Results]The positive detection rate of citrus huanglongbing(HLB)was recorded at 3%,indicating an increase in attention towards this disease compared to 2013.Additionally,the positive detection rate for citrus bacterial canker disease(CBCD)was found to be 16.5%.It was observed that the majority of nurseries with positive samples employed open field rearing practices without the use of mesh chambers,and the primary source of scions was self-propagation.The detection rate of citrus tristeza virus(CTV)was found to be the highest,with a positive detection rate of 63%,and the prevalence in disease-bearing nurseries reached as high as 90%.In comparison to 2013,there had been no improvement in the condition of seedlings affected by CTV.The positive detection rate of citrus yellow vein clearing virus(CYVCV)was found to be 38%,with 70%of the surveyed nurseries exhibiting the disease.The citrus varieties identified as carriers of the disease included‘Qicheng’,‘Shatangju’,‘Wogan’,and‘Gonggan’.Nematodes were isolated from the matrix and roots of seedlings grown in both container and open field environments.The susceptibility of container seedlings to nematodes was found to be 36.4%,while the susceptibility of open field seedlings was 38.6%.Statistical analysis indicated no significant difference in susceptibility between the two groups.[Conclusion

关 键 词：Citrus diseases and pests Investigation qPCR RT-PCR DETECTION 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]