TaEMF2调控小麦抽穗期的功能分析  

Functional analysis of TaEMF2 in regulating wheat heading date

作　　者：武丽芬 夏川[2] 张立超[2] 孔秀英[2] 陈景堂 刘旭 WU Li-Fen;XIA Chuan;ZHANG Li-Chao;KONG Xiu-Ying;CHEN Jing-Tang;LIU Xu(College of Agronomy,Hebei Agricultural University/Hebei Sub-Center for National Maize Improvement Center/State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071001,Hebei,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/State Key Laboratory of Crop Gene Resources and Breeding,Beijing 100081,China;College of Agronomy,Qingdao Agricultural University,Qingdao 266109,Shandong,China)

机构地区：[1]河北农业大学农学院/国家玉米改良中心河北分中心/华北作物改良与调控国家重点实验室,河北保定071001 [2]中国农业科学院作物科学研究所/作物基因资源与育种全国重点实验室,北京100081 [3]青岛农业大学农学院,山东青岛266109

出　　处：《作物学报》2024年第12期2940-2949,共10页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2022YFF1002902)资助。

摘　　要：适宜的抽穗期(开花时间)是作物获得高产和稳产的重要育种目标,EMF2是参与成花调控过程中的重要基因之一,但该基因在小麦中的功能仍不清楚。本研究从普通小麦中克隆了水稻OsEMF2b的直系同源基因TaEMF2,TaEMF2-2D是一个细胞核和细胞质定位蛋白。敲除TaEMF2会推迟小麦的抽穗期,过表达TaEMF2-2D会使小麦抽穗期提前。利用RT-qPCR检测小麦开花相关基因在TaEMF2转基因株系中的表达,结果表明,VRN1和VRN3在敲除转基因株系中的表达水平显著下调,在过表达株系中这2个基因的表达量则显著上调,表明TaEMF2可能通过调控VRN1和VRN3的表达来影响小麦的抽穗期。A suitable heading date(flowering time)is a crucial breeding goal for achieving high and stable crop yields.EMF2 is an important gene involved in the regulation of heading date,but its function in wheat remains unclear.In this study,TaEMF2-2D,the orthologous gene of the rice heading date regulatory gene OsEMF2b,was cloned from wheat.Subcellular localization assays revealed that TaEMF2-2D is a nucleus and cytoplasm protein.To elucidate the role of TaEMF2 in regulating wheat heading date,we used the CRISPR/Cas9 gene editing system to knockout TaEMF2,which resulted in a delayed heading date in wheat.Conversely,overexpression of TaEMF2-2D caused an early heading date.Further analysis showed that the expression levels of the flowering-related genes VRN1 and VRN3 were significantly reduced in the knockout lines,while their expression levels were significantly increased in the overexpression plants,as determined by RT-qPCR.These results suggest that the TaEMF2 gene influences wheat heading date by regulating the expression levels of VRN1 and VRN3.

关 键 词：小麦 抽穗期 TaEMF2 CRISPR/Cas9基因编辑 过表达 

分 类 号：S512.1[农业科学—作物学]