代谢组和转录组分析籼稻福香占干旱胁迫的分子响应  

Metabolome and transcriptome analysis reveal molecular response to drought stress in indica rice Fuxiangzhan

作　　者：王颖姮[1,2] 崔丽丽 蔡秋华[1,2] 林强[1,2] 吴方喜[1,2] 陈飞鹤 谢鸿光[1,2] 朱永生[1,2] 陈丽萍[1,2] 谢华安 张建福[1,2] WANG Ying-Heng;CUI Li-Li;CAI Qiu-Hua;LIN Qiang;WU Fang-Xi;CHEN Fei-He;XIE Hong-Guang;ZHU Yong-Sheng;CHEN Li-Ping;XIE Hua-An;ZHANG Jian-Fu(Rice Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350019,Fujian,China;Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China,Ministry of Agriculture and Rural Affairs/Fuzhou Branch,National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences&Technology/Base of South China,National Key Laboratory of Hybrid Rice/National Rice Engineering Laboratory of China,Fuzhou 350003,Fujian,China)

机构地区：[1]福建省农业科学院水稻研究所,福建福州350019 [2]农业农村部华南杂交水稻种质创新与分子育种重点实验室/福州(国家)水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室/福建省作物种质创新与分子育种省部共建国家重点实验室培育基地/杂交水稻国家重点实验室华南研究基地/水稻国家工程研究中心,福建福州350003

出　　处：《作物学报》2024年第12期2998-3012,共15页Acta Agronomica Sinica

基　　金：福建省自然科学基金(2022J01451);福建省人民政府-中国农业科学院协同创新工程“5511”项目(XTCXGC2021001);福建省财政专项-福建省农业科学院科技创新团队(CXTD2021001)资助。

摘　　要：干旱是影响农业生产最重要的原因之一。本研究以优质香稻福香占为材料,对其苗期干旱响应的生理指标、激素代谢物及基因表达调控网络进行分析。干旱处理后,福香占干旱存活率、抗氧化酶活性高于恢复系明恢63、明恢86和干旱敏感的丽江新团黑谷,但电导率较低且过氧化物累积较少。IAA、ICA、ABA、cZ和SA等5种激素的代谢物含量上升,tZ、DHZ、GA1、JA等11种激素的代谢物含量下降。6118个差异表达基因(DGEs)中有2615个上调、3503个下调,涉及了光合作用、能量代谢、转录调控、氧化还原、离子结合等生物学过程和氨基酸、糖、脂肪酸、激素等合成代谢及植物激素信号转导等相关途径。激素代谢组和转录组学分析同时鉴别出植物激素信号转导、玉米素生物合成、类胡萝卜素生物合成和色氨酸代谢4个KEGG代谢通路,并构建了差异表达基因调控网络。转录因子、抗氧化酶基因、渗透调节等相关的28个干旱应答关键基因在福香占干旱处理后的表达量均上调。福香占受干旱胁迫后,激素水平发生了变化;抗逆应答的基因表达量均上调,包括转录因子、抗氧化系统基因、渗透调节及其他耐旱基因;抗氧化酶活性相关生理指标发生改变。研究结果有助于进一步挖掘抗旱基因,服务水稻抗旱育种。Drought is one of the most significant factors affecting agricultural production.In this study,the physiological indexes,hormone metabolites,and gene expression regulation network of drought response were analyzed for the high grain quality fragrant rice Fuxiangzhan.After drought stress,Fuxiangzhan exhibited higher drought survival rates and antioxidant enzyme activity compared to restorer lines Minghui63,Minghui86,and the drought-sensitive line Lijiangxintuanheigu,while showing lower membrane iron leakage and less peroxide accumulation.Five hormone metabolites(IAA,ICA,ABA,cZ,and SA)increased,whereas 11 hormone metabolites,including tZ,DHZ,GA1,and JA,decreased.A total of 6118 differentially expressed genes(DEGs)were identified,including 2615 up-regulated and 3503 down-regulated genes,which are involved in biological processes such as photosynthesis,energy metabolism,transcriptional regulation,REDOX,and ion binding,as well as molecular functions related to amino acids,sugars,fatty acids,hormones,and other anabolic metabolism and plant hormone signal transduction.KEGG pathways involving plant hormone signal transduction,zein biosynthesis,carotenoid biosynthesis,and tryptophan metabolism were identified from hormone metabolites and transcriptome analysis.Differentially expressed gene regulatory networks of the four pathways were constructed.The expression levels of 28 drought response genes related to transcription factors,antioxidant enzymes,and osmotic regulation were all up-regulated after drought stress in Fuxiangzhan.Our conclusion is that the hormone levels in Fuxiangzhan change after drought stress.The expression of anti-stress genes,including transcription factors,antioxidant system genes,osmoregulation,and other drought tolerance genes,were up-regulated.These changes lead to alterations in the activity of antioxidant enzymes and other physiological indexes.These results are helpful for further exploration of drought-resistant genes and serve in rice drought resistance breeding.

关 键 词：福香占 抗旱 生理特性 激素代谢组 调控网络 

分 类 号：S566.1[农业科学—作物学]