小麦籽粒大小相关基因TaCYP78A17的功能标记开发  

Development of functional markers of wheat grain size related gene TaCYP78A17

作　　者：张天星 李梦 吴林楠 赵惠贤[2] 胡胜武[1] 马猛[2] ZHANG Tian-Xing;LI Meng;WU Lin-Nan;ZHAO Hui-Xian;HU Sheng-Wu;MA Meng(Collage of Agronomy,Northwest A&F University,Yangling 712100,Shaanxi,China;College of Life Science,Northwest A&F University,Yangling 712100,Shaanxi,China)

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《作物学报》2024年第12期3025-3034,共10页Acta Agronomica Sinica

基　　金：陕西省重点研发项目(2021NY-079);国家自然科学基金项目(32072059,32072003)资助。

摘　　要：籽粒大小是影响小麦产量的重要因素之一。为了开发小麦籽粒大小相关的功能标记,本研究克隆得到一个潜在籽粒大小相关基因TaCYP78A17,并对其进行了系统进化、表达模式、等位变异分析和功能标记开发。结果表明,TaCYP78A17基因是小麦细胞色素P450 CYP78A家族的一员,且在小麦幼穗和籽粒中高表达;在30份普通小麦品种中发现TaCYP78A17-Ap存在6个SNP和2个InDel;根据InDel 4和InDel 8开发功能标记InDel-A17,可将30份普通小麦品种分为TaCYP78A17-Ap-HapⅠ, TaCYP78A17-Ap-HapⅡ和TaCYP78A17-Ap-HapⅢ三种单倍型;利用该功能标记在323份普通小麦品种中进行验证,发现该功能标记可以有效区分上述3种单倍型;表型调查发现具有TaCYP78A17-Ap-HapI单倍型的小麦其千粒重和籽粒大小显著高于具有TaCYP78A17-Ap-HapⅡ或TaCYP78A17-Ap-HapⅢ单倍型的小麦。研究结果有望应用于小麦分子标记辅助选择育种。Grain size is a crucial factor influencing wheat yield.To develop functional markers related to wheat grain size,we cloned a potential grain size-related gene,TaCYP78A17,and conducted systematic evolution,expression pattern,allelic variation,and functional marker development studies.The results revealed that TaCYP78A17 belongs to the wheat cytochrome P450 CYP78A family and is highly expressed in wheat spikes and grains.Six SNPs and two InDels were identified in the TaCYP78A17-Ap among thirty common wheat varieties.A functional marker,InDel-A17,was developed based on InDel 4 and InDel 8,classifying the thirty wheat varieties into three haplotypes:TaCYP78A17-Ap-HapI,TaCYP78A17-Ap-HapⅡ,and TaCYP78A17-Ap-HapⅢ.This functional marker was validated in 323 wheat varieties,demonstrating its ability to effectively distinguish the three haplotypes.Phenotypic investigations indicated that wheat with the TaCYP78A17-Ap-HapⅠ haplotype exhibited significantly higher thousand-grain weight and larger seed size compared to those with TaCYP78A17-Ap-HapⅡ or TaCYP78A17-Ap-HapⅢ.These findings hold promise for the application of molecular marker-assisted selection in wheat breeding.

关 键 词：小麦 籽粒大小 TaCYP78A17 功能标记 

分 类 号：S512.1[农业科学—作物学]