花生转录因子基因AhWRI1的克隆及表达分析  

Cloning and expression analysis of transcription factor AhWRI1s in peanut

作　　者：殷祥贞 赵健鑫 郝翠翠 潘丽娟[1] 陈娜[1] 许静 姜骁 赵旭红 王恩琪 曹欢 禹山林[1] 迟晓元[1] YIN Xiang-Zhen;ZHAO Jian-Xin;HAO Cui-Cui;PAN Li-Juan;CHEN Na;XU Jing;JIANG Xiao;ZHAO Xu-Hong;WANG En-Qi;CAO Huan;YU Shan-Lin;CHI Xiao-Yuan(Shandong Peanut Research Institute,Qingdao 266100,Shandong,China;College of Marine Science and Biological Engineering,Qingdao University of Science and Technology,Qingdao 266042,Shandong,China)

机构地区：[1]山东省花生研究所,山东青岛266100 [2]青岛科技大学海洋科学与生物工程学院,山东青岛266042

出　　处：《作物学报》2024年第12期3155-3164,共10页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13);山东省农业科学院农业科技创新工程项目(CXGC2023F20,CXGC2024F20);广东省重点领域研发计划项目(2020B020219003);新疆维吾尔自治区重大科技专项(2022A02008-3);泰山学者工程专项,山东省重点研发计划(农业良种工程)项目(2022LZGC007);山东省自然科学基金项目(ZR2021QC172,ZR2023QC146);山东省重点研发计划(乡村振兴科技创新提振行动计划)项目(2022TZXD0031)资助。

摘　　要：花生是世界范围内广泛栽培的油料和经济作物之一,由于其高油脂和高蛋白质含量,已成为人们主要的油脂和蛋白质来源。随着世界对植物油需求的不断增加,改良花生脂肪酸组成和提高油脂含量成为花生育种工作的重要内容。转录调控因子可调控油脂合成相关代谢途径中一系列基因的表达,显著影响油脂合成和代谢。本研究从花生品种花育33号的叶片中克隆得到2个转录因子基因AhWRI1-1和AhWRI1-2,AhWRI1-1的ORF为1101 bp,编码366个氨基酸;AhWRI1-2的ORF为1128 bp,编码375个氨基酸。生物信息学分析发现,AhWRI1-1和AhWRI1-2均含有2个AP2/EREBP结构域。利用qRT-PCR检测AhWRI1-1和AhWRI1-2在不同组织中的表达模式发现,AhWRI1-1在种子中的表达量最高,可能参与调节脂肪酸合成和油脂积累;AhWRI1-2在下胚轴中的表达量最高,可能参与下胚轴的发育。此外,AhWRI1-1和AhWRI1-2对非生物胁迫的响应存在差异,表明AhWRI1-1和AhWRI1-2非生物胁迫中的作用也存在差异。通过在酵母中的转录激活试验验证,AhWRI1-1和AhWRI1-2均具有转录激活活性。本研究为以后对AhWRI1-1和AhWRI1-2的功能进行深入研究奠定了基础。Peanut is one of the widely cultivated oil and economic crops worldwide and has become a major source of oil and protein for humans due to its high oil and protein content.With the increasing global demand for vegetable oil,improving the fatty acid composition and increasing the lipid content of peanut seeds has become a top priority in peanut breeding.Transcrip-tional regulators can modulate the expression of a series of genes in metabolic pathways related to lipid synthesis,significantly affecting lipid synthesis and metabolism.In this study,two transcription factors,AhWRI1-1 and AhWRI1-2,were cloned from the leaves of Huayu 33.The ORF of AhWRI1-1 was 1101 bp,encoding 366 amino acids,and the ORF of AhWRI1-2 was 1128 bp,encoding 375 amino acids.Bioinformatics analysis revealed that both AhWRI1-1 and AhWRI1-2 contained two AP2/EREBP conserved domains.The expression patterns of AhWRI1-1 and AhWRI1-2 in different tissues were detected by qRT-PCR.The results showed that AhWRI1-1 had the highest expression in seeds,suggesting its involvement in the regulation of fatty acid syn-thesis and oil accumulation,while AhWRI1-2 had the highest expression in hypocotyls,indicating its role in hypocotyl develop-ment.Additionally,the differences in the responses of AhWRI1-1 and AhWRI1-2 to abiotic stresses suggested that these transcrip-tion factors may play different roles under such conditions.Transcriptional activation experiments in yeast showed that both Ah-WRI1-1 and AhWRI1-2 possess transcriptional activation activities.This study lays the foundation for future in-depth functional studies of AhWRI1-1 and AhWRI1-2.

关 键 词：花生 AP2/EREBP转录因子 非生物胁迫 基因表达分析 转录激活 

分 类 号：S565.2[农业科学—作物学]