荧光偏振成像技术及其生物学应用  

Biological Applications of Fluorescence Polarization Imaging

作　　者：杨子仪 李世晗 刘芷如 钟素艺 李美琪 席鹏[1] Yang Ziyi;Li Shihan;Liu Zhiru;Zhong Suyi;Li Meiqi;Xi Peng(Department of Biomedical Engineering,Peking University,Beijing 100871,China;Department of Mechanics and Engineering Science,Peking University,Beijing 100871,China;School of Life Sciences,Peking University,Beijing 100871,China)

机构地区：[1]北京大学生物医学工程系,北京100871 [2]北京大学力学与科学工程系,北京100871 [3]北京大学生命科学学院,北京100871

出　　处：《激光与光电子学进展》2024年第18期1-15,共15页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(62335008)。

摘　　要：荧光偏振成像通过测量样品中荧光分子的偏振状态,得到样品的取向结构特征,在生物学领域有着广泛的应用。偏振成像常与其他荧光显微技术结合,用来解析细胞结构的排列方向、生物大分子的实时动态取向和组织的有序性等信息,以研究细胞的生理过程、药物对细胞的作用及异常结构的检测。本文阐述了荧光偏振成像在生物学等领域的应用,对利用偏振信息获取不同样品取向结构的研究方法进行分析比较,并对其未来发展趋势进行展望。Fluorescence polarization imaging can obtain the orientation and structural features of the sample by measuring the polarization parameters of fluorescent molecules,owing to which it is widely used in biology research.Moreover,it is often combined with other fluorescence microscopy techniques to analyze the arrangement of cellular structures,the realtime dynamic orientation of biomolecules,and the organization of tissues.This approach enables research on cellular physiological processes,the effects of drugs on cells,and the detection of abnormal structures.In this study,we focus on the applications of fluorescence polarization imaging in biology research,including the analysis and comparison of different methods that utilize polarization information to obtain orientation structures of different samples.Furthermore,we provide insights into future development trends in this field.

关 键 词：荧光偏振成像 分子取向解析 生物显微成像 

分 类 号：Q-336[生物学]