半夏HD-Zip基因家族全基因组鉴定及表达分析  

Genome Wide Identification and Expression Analysis of the HD-Zip Gene Family in Pinellia ternata

作　　者：刘梦梦 刘晓 尤倩 伯晨 朱艳芳[1] 段永波[1] 薛建平[1] 王德信 薛涛[1] LIU Meng-Meng;LIU Xiao;YOU Qian;BO Chen;ZHU Yan-Fang;DUAN Yong-Bo;XUE Jian-Ping;WANG De-Xin;XUE Tao(College of Life Sciences/Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants,Huaibei Key Laboratory of Efficient Cultivation and Utilization of Resource Plants,Huaibei Normal University,Huaibei 235000,China;College of Agriculture and Engineering,Heze University,Heze 274009,China)

机构地区：[1]淮北师范大学生命科学学院/安徽省特色资源植物利用工程实验室,资源植物高效栽培与利用淮北市重点实验室,淮北235000 [2]菏泽学院农业与生物工程学院,菏泽274015

出　　处：《农业生物技术学报》2024年第11期2540-2551,共12页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(82274048);安徽省高校自然科学研究项目(KJ2021A0532);安徽省高校优秀创新团队(2022AH010029;2023ZK016;PT21110037)。

摘　　要：环境胁迫引发的半夏(Pinellia ternata)倒苗,严重限制了其产业的发展,同源异型域-亮氨酸拉链蛋白(homeodomain-leucine zipper,HD-Zip)家族基因广泛参与植物的逆境响应调控。为发掘参与半夏倒苗调控的HD-Zip基因,本研究开展了半夏基因组水平HD-Zip家族基因的鉴定分析。结果发现,半夏中共鉴定出17个HD-Zip家族基因,其中15个成员挂载至9条染色体上。进化分析显示,鉴定出的17个HD-Zip家族成员包括9个HD-ZipⅠ亚家族成员和8个HD-ZipⅡ亚家族成员,蛋白序列中均具有高度保守性的HD结构域(WFQNRR)。亚细胞定位预测显示,17个HD-Zip家族成员均定位于细胞核中;共线性分析表明,8个PtHDZ基因形成4对同源基因对,且1个基因与水稻(Oryza sativa)之间存在共线性关系。顺式作用元件预测分析表明,鉴定的PtHDZ基因启动子均含激素、胁迫响应元件和转录因子结合位点等元件。组织表达模式分析显示,半夏HDZ家族基因在不同组织中呈现差异表达,多数基因在根和块茎中高表达;高温胁迫下表达模式分析发现,14个PtHDZs受高温诱导表达,2个PtHDZs的表达受高温抑制。本研究为解析半夏的倒苗机理及分子育种提供了基因资源。The sprout tumble(ST)of Pinellia ternata caused by environmental sress severely limits its industrial development,and the homeodomain-leucine zipper(HD-Zip)family genes are widely involved in the regulation of plant stress response.To explore the HD-Zip genes involved in the regulation of ST in P.ternata,the HD-Zip family genes of P.ternata were identified and analyzed at the genome level in this study.It was found that 17 HD-Zip family genes were identified in P.ternata,among them,15 of which were attached to 9 chromosomes.Evolutionary analysis showed that the identified 17 HD-Zip family members included 9 HD-ZipⅠsubfamily members and 8 HD-ZipⅡsubfamily members,all of which had highly conserved HD domains(WFQNRR)in their protein sequences.Subcellular localization prediction showed that all 17 members of the HD-Zip family were located in the nucleus,and collinearity analysis showed that 8 PtHDZ genes formed 4 pairs of homologous gene pairs,and 1 genes had a collinearity relationship with rice(Oryza sativa).The elements of hormones response,stress response,and transcription factor binding sites were contained in the promoters of PtHDZ genes via the prediction analysis of cis acting elements.Organizational expression pattern analysis revealed that the PtHDZ family genes showed differential expression in different tissues,most of which were highly expressed in roots and tubers.And the expression of 14 PtHDZs were found to be induced while 2 PtHDZs were inhibited by high temperature.This study provides genetic resources for analyzing the mechanism of ST and molecular breeding in P.ternata.

关 键 词：半夏 转录因子 HD-Zip家族 高温胁迫 基因表达 

分 类 号：S609.9[农业科学—园艺学]