健康成年公鸭睾丸大小比较及其相关调控基因表达分析  

Comparison of the Size of Testes in Healthy Adult Male Duck(Anas platyrhynchos)and Analysis of the Expression of Related Regulatory Genes

作　　者：朱春红[1] 陶志云[1] 王志成 宋卫涛[1] 刘宏祥[1] 章双杰[1] 徐文娟[1] 顾昊天 王逸飞 李慧芳[1] ZHU Chun-Hong;TAO Zhi-Yun;WANG Zhi-Cheng;SONG Wei-Tao;LIU Hong-Xiang;ZHANG Shuang-Jie;XU Wen-Juan;GU Hao-Tian;WANG Yi-Fei;LI Hui-Fang(Jiangsu Institute of Poultry Science,Yangzhou 225125,China)

机构地区：[1]江苏省家禽科学研究所,扬州225125

出　　处：《农业生物技术学报》2024年第11期2603-2613,共11页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2022YFD1300104);扬州市现代农业项目(YZ2022050);江苏省现代农业(水禽)产业技术体系(JATS[2022]404)。

摘　　要：本研究旨在通过对健康成年公鸭(Anas platyrhynchos)睾丸大小和产精液量差异个体睾丸组织进行转录组测序和生物信息学分析,筛选出与睾丸大小以及生精能力相关的差异表达基因(differentially expressed genes,DEGs)及信号通路,为公鸭睾丸功能性选育提供数据支撑。本研究选用230日龄健康成年公鸭40只,根据睾丸称重结果分组睾丸轻组(GS)和睾丸重组(GL),根据精液采集量多少分组产精液量少组(GF)和精液量多组(GM),各组分别采集睾丸组织利用BGIseq平台进行转录组mRNA测序,对筛选到的DEGs进行GO和KEGG功能注释和富集分析,qRT-PCR验证挑选的DEGs表达水平。结果显示,体重差异不显著公鸭中,部分个体睾丸大小有显著性差异;睾丸大小差异不显著公鸭中,人工采精训练后采集精液量有显著差异。睾丸重差异组和精液量差异组共分析到383个DEGs,不同分组间共同DEGs数为36个,qRT-PCR结果显示,转录组测序结果准确可靠。GO和KEGG分析结果表明,DEGs显著富集于信号转导、细胞膜、膜的整体组成、细胞质膜、G蛋白偶联受体活性、细胞分裂正向调节、cAMP信号、PI3KAkt信号、钙信号等相关通路,GPR50、GPR132、GRER1、IGF1、TACR3、HTR1F、FGF19、FGF3、GABRG1、ADCY1等基因富集其中,可能与睾丸功能紧密相关。本研究初步筛选了公鸭睾丸大小以及生精能力差异的基因,为水禽睾丸功能的遗传育种提供了的理论依据。This study was aim to analyze the testis tissue from healthy adult male ducks(Anas platyrhynchos)with differences in testicular size and semen production by RNA-seq and bioinformatics.The differentially expressed genes(DEGs)and signaling pathways related to testicular size and spermatogenesis were screened to provide data for supporting male duck breeding.In this study,40 male ducks(230-day-old)were used to collect testes tissues according to testis size(light weight of testes,GS;heavy weight of testes,GL)and semen production(less semen volume,GF;excessive semen volume,GM).Collect the testicular tissues and use the BGIseq platform for transcriptomic mRNA sequencing,GO and KEGG functional annotation and enrichment analysis were used to analyze the candidate DEGs.The expression of DEGs were verified by qRT-PCR.The results showed that there was a significant difference in testicular size among male ducks with no significant difference in body weight,there was also a significant difference in the production of semen after artificial training.A total of 383 DEGs were analyzed in different semen production and different testis size groups,and 36 DEGs were screened among different groups.The results of qRT-PCR showed that the result of transcriptome sequencing was accurate and reliable.GO and KEGG analysis showed that DEGs was significantly enriched in signal transduction,cell membrane,membrane composition,plasma membrane,G protein-coupled receptor activity,positive regulation of cell division and cAMP signal,pI3K-AKT signal,calcium signal pathway and other related pathways,genes GPR50,GPR132,GRER1,IGF1,TACR3,HTR1F,FGF19,FGF3,GABRG1,ADCY1 were enriched in these pathways,which might be closely related to testicular function.This study screened the genes related different testicular size and spermatogenic capacity in ducks,which provides a theoretical basis for the genetic breeding of testicular function.

关 键 词：鸭 转录组测序 睾丸 精液量 差异表达基因 

分 类 号：S835[农业科学—畜牧学]