舒芬太尼对骨质疏松大鼠氧化应激损伤的影响及有关机制研究  

Effect of sufentanil on oxidative stress injury in osteoporotic rats and its related mechanisms

作　　者：徐海华 华汤锋 沈志恩 庄伟 Xu Haihua;Hua Tangfeng;Shen Zhien;Zhuang Wei(Department of Anesthesiology,Jiangnan Hospital Affiliated to Binjiang College,Zhejiang Hospital of Traditional Chinese Medicine University,Hangzhou 311200,CHina)

机构地区：[1]浙江中医药大学滨江学院附属江南医院麻醉科,杭州311200

出　　处：《中华内分泌外科杂志（中英文）》2024年第5期696-701,共6页Chinese Journal of Endocrine Surgery

基　　金：萧山区重大科技项目(2019222)。

摘　　要：目的探讨舒芬太尼(sufentanil,Suf)对骨质疏松(osteoporosis,OP)大鼠氧化应激损伤的影响及机制。方法大鼠按照随机数字表法随机分为正常组、OP组、低浓度Suf组、高浓度Suf组、阿仑膦酸钠组、高浓度Suf+AMPK抑制剂(dorsomorphin)组,每组12只。正常组大鼠仅摘除卵巢周围的少许脂肪组织,除正常组外,其他组大鼠均通过切除双侧卵巢的方法构建OP模型,建模成功后给药,给药1次/d,持续12周。微型CT扫描仪测量大鼠骨密度及骨小梁结构参数骨小梁数量、骨小梁厚度、骨小梁分离度;ELISA检测大鼠血清中碱性磷酸酶(alkaline phosphatase,ALP)、骨钙素、核因子KB受体活化因子配体(ligand of receptor activator of NF-kB,RANKL)、骨保护素(osteoprotegerin,OPG)水平;HE染色检测股骨组织的病理变化;试剂盒检测股骨组织中丙二醛(malondialdehyde,MDA)、活性氧(reactive oxygen species,ROS)、超氧化物歧化酶(superoxide dismutase,SOD)水平;Western blot检测股骨组织中磷酸化腺苷酸活化蛋白激酶(phosphorylated amp-activated protein kinase,p-AMPK)、过氧化物酶体增殖物激活受体γ辅激活因子1α(peroxisome proliferator-activated receptorγcoactivator 1α,PGC-1α)、沉默信息调节因子2相关酶1(silent information silent information regulation 2homolog-1,SIRT1)蛋白。结果与正常组相比,OP组大鼠骨小梁结构薄散,骨小梁数量少,骨小梁间隙增宽,以杆状为主,骨密度、骨小梁数量、骨小梁厚度、血清中ALP、骨钙素、OPG水平、股骨组织中SOD水平及p-AMPK、PGC-1α、SIRT1蛋白降低(q=38.00,67.81,47.91,48.32,28.42,21.23,52.22,30.86,24.11,24.55,P均<0.001),骨小梁分离度、血清中RANKL水平、股骨组织中MDA、ROS水平升高(q=42.00,37.97,50.56,72.01,P均<0.001);与OP组相比,低浓度Suf组、高浓度Suf组、阿仑膦酸钠组大鼠骨小梁数量增加,间隙减少,骨小梁结构逐步恢复到板状,骨密度、骨小梁数量、骨小梁厚度、血清中ALP、�ObjectiveTo investigate the effect and mechanism of Sufentanil(Suf)on oxidative stress injury in osteoporosis(OP)rats.MethodsRats were randomly separated into normal group,OP group,low concentration Suf group,high concentration Suf group,alendronate sodium group,and high concentration Suf+AMPK inhibitor(Dorsomorphin)group,with 12 rats in each group.The normal group of rats only removed a small amount of adipose tissue around the ovaries.Except for the normal group,rats in other groups constructed OP models by removing both ovaries.After successful modeling,they were administered once a day for 12 weeks.Micro CT scanners were applied to measure bone density and trabecular structure parameters in rats,including trabecular quantity,trabecular thickness,and trabecular separation.ELISA was applied to detect levels of osteocalcin,alkaline phosphatase(ALP),osteoprotegerin(OPG),and receptor activator of nuclear factor kappa-B ligand(RANKL)in rat serum.HE staining was applied to detect pathological changes in femoral tissue.Reagent kit was applied to detect levels of malondialdehyde(MDA),reactive oxygen species(ROS),and superoxide dismutase(SOD)in femoral tissue.Western blot analysis of phosphorylated adenylate activated protein kinase(p-AMPK),peroxisome proliferator-activated receptor-γ-coactivator 1α(PGC-1α),silence-information regulatory factor2-associated enzyme 1(SIRT1)proteins in femur tissue.ResultsCompared with the normal group,the bone trabecular structure of rats in the OP group was thin and scattered,with fewer bone trabeculae and widened trabecular gaps,mainly in the form of rods,the bone density,number of trabeculae,thickness of trabeculae,levels of ALP,osteocalcin,OPG in serum,the level of SOD,and p-AMPK,PGC-1α,and SIRT1 proteins in femoral tissue decreased(q=38.00,67.81,47.91,48.32,28.42,21.23,52.22,30.86,24.11,24.55,P all<0.001),the bone trabecular separation,level of RANKL in serum,and the levels of MDA and ROS in femoral tissue increased(q=42.00,37.97,50.56,72.01,P all<0.001).Compared with the OP gro

关 键 词：骨质疏松 舒芬太尼 氧化应激 股骨 

分 类 号：R614[医药卫生—麻醉学]