宫颈癌与TRAIL基因多态性及其肿瘤标志物的相关性研究  

STUDY ON THE CORRELATION BETWEEN CERVICAL CANCER AND TRAIL GENE POLYMORPHISM AND ITS TUMOR MARKERS

作　　者：闫晓华 彭宝相 程振娜 刘瑞磊[3] 张娟 YAN Xiao-hua;PENG Bao-xiang;CHENG Zhen-na;LIU Rui-lei;ZHANG Juan(Shandong Medical College,Linyi,China 276000;Linyi Cancer Hospital;Linyi People's Hospital)

机构地区：[1]山东医学高等专科学校,山东临沂276000 [2]临沂市肿瘤医院 [3]临沂市人民医院

出　　处：《山东医学高等专科学校学报》2024年第5期7-9,共3页Journal of Shandong Medical College

基　　金：山东省医药卫生科技发展计划项目(No.202205010687)。

摘　　要：目的探讨TRAIL基因多态性及其血清水平与宫颈癌发生及肿瘤标志物的相关性。方法收集宫颈癌患者92例为宫颈癌组,健康者90例为对照组,提取两组全血基因组DNA,用PCR-RFLP和测序法分析TRAIL基因第5外显子3′-UTR 1525G/A、1588G/A、1595C/T基因多态性;用ELISA法检测血清sTARIL水平。结果两组1525G/A、1588G/A、1595C/T基因型分布有统计学意义(P<0.05);宫颈癌组1525G、1588G、1595C等位基因频率明显高于对照组(P<0.05),血清sTRAIL水平明显低于对照组(P<0.05),血清sTRAIL水平与血清CEA、CA125、SCC水平没有相关性。结论宫颈癌存在TRAIL易感基因,其血清TRAIL水平降低。Objective To investigate the genetic polymorphisms of TRAIL and its serum level with cervical cancer occurrence and tumor markers.Methods A total of 92 cervical cancer patients and 90 healthy controls were selected.The genomic DNA was extracted from whole blood,and the polymorphisms of the 3′-UTR of TRAIL gene at 1525G/A,1588G/A,and 1595C/T were analyzed by methods of PCR-RFLP and sequencing.The serum sTRAIL level was detected by ELISA.Results The genotype distribution of 1525G/A,1588G/A,and 1595C/T in the cervical cancer group was statistically different from that in the control group(P<0.05);the frequency of 1525G,1588G,and 1595C alleles in the cervical cancer group was significantly higher than that in the control group(P<0.05);the serum sTRAIL level in the cervical cancer group was significantly lower than that in the control group(P<0.05);there was no statistically significant correlation between the serum sTRAIL level and the serum CEA,CA125,and SCC.Conclusion Cervical cancer has TRAIL susceptible genes,and the serum TRAIL levels are reduced.

关 键 词：肿瘤坏死因子相关凋亡诱导配体 基因多态性 宫颈癌 

分 类 号：R737.3[医药卫生—肿瘤]