基于纳米多孔阳极氧化铝传感基底的反射干涉光谱技术灵敏度研究  

Study on the Reflection Interference Spectroscopy Sensitivity Based on Nanoporous Anodic Alumina Sensing Substrate

作　　者：王健飞 王凯歌[1] WANG Jianfei;WANG Kaige(Key Laboratory of Photoelectric Technology of Shaanxi Province,National Center for International Research of Photoelectric Technology&Nano-Functional Materials,Cultivation Base of State Key Laboratory of Optoelectronictechnology and Functional Materials of the Ministry and Shaanxi Province,Institute of Photonics and PhotonTechnology,Northwest University,Xi'an 710127,China)

机构地区：[1]陕西省光电技术重点实验室,国家级光电技术与纳米功能材料国际联合研究中心,省部共建光电技术与功能材料国家重点实验室培育基地,西北大学光子学与光子技术研究所,西安710127

出　　处：《光子学报》2024年第10期158-168,共11页Acta Photonica Sinica

基　　金：国家自然科学基金面上项目(Nos.62275216,61775181);陕西省自然科学基础研究计划-重大基础研究项目和陕西省科技创新团队项目(Nos.TZ0393,S2018-ZC-TD-0061);国家自然科学基金国家重大科研仪器研制项目(No.51927804)。

摘　　要：为了明确以多孔薄膜为传感基底的反射干涉光谱技术在信号强度、检测灵敏度和判断准确性方面的特性,本研究结合实验和仿真计算,定量分析了影响灵敏度的各种条件及其作用机理。首先制备了孔径相同(80 nm)、2种孔深不同(9μm、11μm)的纳米多孔阳极氧化铝薄膜。实测表明它们对填充液的反射干涉光谱检测灵敏度分别达到了5.60×10^(3) nm/RIU、8.00×10^(3) nm/RIU。其次,构建了以纳米多孔阳极氧化铝为传感基底的反射干涉光谱物理模型,仿真结果显示当待测样品的折射率变化范围为1.00~1.60时,对于孔径为80 nm、孔深为9μm的传感基底,其灵敏度为6.20×10^(3) nm/RIU,当孔深增加为11μm时,灵敏度为8.30×10^(3) nm/RIU。实验与仿真结果具有高度一致性,孔径相同时,孔深越大,灵敏度越高。该研究方法与结果,对于以纳米多孔阳极氧化铝为传感基底的反射干涉光谱技术在原位监测生物化学药物单分子特性及其相互作用性质等方面有促进作用。Reflective Interference Spectroscopy(RIf S)technology based on porous thin film as a sensor substrate provides many advantages for biomedical molecular characterization,among which detection sensitivity is crucial.Nanoporous Anodic Aluminum(Np AA),as an effective nanostructured sensor platform,combined with RIf S technology,has practical applications in studying biomolecular properties and interactions between drug molecules and biomolecules.However,challenges will be faced,when the sample quantity is small and the refractive index of solution changes slightly,which leading to issues such as weak intensity of RIf S spectral signal,insufficient detection sensitivity,and inaccurate in judgment results.In order to improve the practicality of the reflective interference spectroscopy based on nanoporous thin films in the detection of trace samples,a model is established in this study,and various conditions affecting sensitivity and mechanisms are quantitatively investigated and analyzed by combining experiment and simulation.First,two types of Np AA sensor substrates with same pore diameter(80 nm)but different pore depths(9μm and 11μm)were prepared with the standard two-step anodization method with oxalic acid as the electrolyte.The morphology of Np AA was characterised with scanning electron microscope,revealing well-ordered pore structures with vertical and distinct pore walls.The material has a high porosity,with significant gaps between the pores and a periodic arrangement.This pore structure enhances the film substrate's light field capture,creating standing wave phenomenon and significantly boosting the sensing signal strength.Different concentrations of glycerol solutions,with refractive indices ranging from 1.33 to1.60,were then applied to the two types of Np AA sensor substrates.After the solution fully penetrated into the pores,the measurement was performed.The measurement data indicated that the sensitivity of RIf S for detecting trace amounts of filling liquid is 5.60×10^(3) nm/RIU for a 9μm depth and

关 键 词：纳米多孔阳极氧化铝 生物单分子传感器 反射干涉光谱 传感基底 灵敏度 

分 类 号：O435.1[机械工程—光学工程] O433.4[理学—光学]