小分子化学污染物广谱识别性核酸适配体的制备及其应用进展  

Preparation and Application Progress of Broad-Spectrum Recognition Nucleic Acid Aptamers for Small Molecular Chemical Pollutants

作　　者：陈莹杰 王佳琦 宋岳龙 贾竹青 李德喜[1] 王战辉[2] 杜向党[1] 李成龙 CHEN Yingjie;WANG Jiaqi;SONG Yuelong;JIA Zhuqing;LI Dexi;WANG Zhanhui;DU Xiangdang;LI Chenglong(International Joint Research Center of National Animal Immunology,Ministry of Education Key Laboratory for Animal Pathogens and Biosafety,Ministry of Agriculture and Rural Affairs Key Laboratory of Quality and Safety Control of Poultry Products,College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China;National Key Laboratory of Veterinary Public Health and Safety,Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)

机构地区：[1]河南农业大学动物医学院国家动物免疫学国际联合研究中心动物病原与生物安全教育部重点实验室农业农村部禽类产品质量安全控制重点实验室,河南郑州450046 [2]中国农业大学动物医学院兽医公共卫生安全全国重点实验室动物源食品安全检测技术北京市重点实验室,北京海淀100193

出　　处：《中国兽医杂志》2024年第11期85-97,共13页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(32002342);河南省自然科学基金项目(242300420152)。

摘　　要：作为新型生物识别材料,广谱识别性核酸适配体在食品和环境中小分子化学污染物及其结构类似物的同时检测中有很大的应用潜力。本文综述了有关腺苷受体拮抗剂、被非法滥用活性物质、抗炎药物、抗微生物药物、工业化学品、农药、类固醇雌激素和毒素等小分子化学污染物广谱识别性核酸适配体的筛选方法和优化策略,归纳总结了109条核酸适配体序列和平衡解离常数(或相对亲和力)及其所用的测定方法,并简要介绍了基于广谱识别性核酸适配体所开发的生物传感器,以期为食品和环境中小分子化学污染物快速检测产品的更新换代提供参考。As a new type of bio-recognition material,broad-spectrum recognition nucleic acid aptamers have great application potential in the simultaneous detection of small molecular chemical pollutants and their structural analogs in food and environment.This paper reviews the screening methods and optimization strategies for broad-spectrum nucleic acid aptamers targeting small molecular chemical pollutants,such as adenosine receptor antagonists,illegally abused active substances,anti-inflammatory drugs,antimicrobial drugs,industrial chemicals,pesticides,steroid estrogens,and toxins.It summarizes 109 nucleic acid aptamer sequences and their equilibrium dissociation constants(or relative affinity)as well as the measurement methods used.Furthermore,biosensors based on broad-spectrum recognition aptamers are briefly introduced,providing a reference for the development and improvement of rapid detection products for small molecular chemical pollutants in food and the environment.

关 键 词：小分子化学污染物 广谱 核酸适配体 生物传感器 

分 类 号：S851[农业科学—预防兽医学] O657[农业科学—兽医学]