机械剪切力对人脂肪组织细胞表型及特性的影响  

Phenotypic and cellular characteristics of adipose tissue prepared using mechanical shear force on human fat

作　　者：叶媛[1] 邹敬江 胡葵葵[1] 姜金豆[1] 郭炳阳 李雄杰 谭梅军 YE Yuan;ZOU Jingjiang;HU Kuikui;JIANG Jindou;GUO Bingyang;LI Xiongjie;TAN Meijun(Department of Plastic and Cosmetic Surgery,Guangdong Women and Children Hospital,Guangzhou 511400,China)

机构地区：[1]广东省妇幼保健院医学美容科,广东广州511400

出　　处：《中国美容整形外科杂志》2024年第10期625-631,共7页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：广州市科技计划项目(202102020179)。

摘　　要：目的分析脂肪胶制备过程中所受的剪切力对最终脂肪成活率的影响。方法自2018年7月至2019年1月,广东省妇幼保健院医学美容科获取吸脂手术患者的脂肪,将其制备成标准的Coleman fat作为对照组,再将Coleman fat进一步利用机械剪切分离,制备成的基质血管片段(stromal vascular fraction,SVF)/细胞外基质(extracellular matrix,ECM)凝胶状(gel)脂肪(SVF/ECM gel)作为实验组。对这两种脂肪成活率、细胞组分及表型进行对比研究。结果裸鼠背部移植后3、14、28、60 d,SVF/ECM gel的成活率显著高于Coleman fat。脂肪的粘度与剪切力成正比。虽然剪切力不影响细胞总数,但它显著减少了活细胞的数量。流式细胞术显示,SVF/ECM gel中间充质干细胞、细胞外膜上脂肪基质细胞和脂肪来源干细胞(adipose-derived stem cells,ADSCs)数量较多,但内皮祖细胞数量较Coleman fat减少。结论机械剪切力分离脂肪可以增加脂肪细胞的多能性,从而在细胞疗法中改善脂肪移植物成活率。Objective To analyze the effect of mechanical shear force on the final fat retention in the preparation of stromal vascular fraction(SVF)/extracellular matrix(ECM)gel from a cytological perspective.Methods Fat aspirates from patients undergoing liposuction were prepared into standard Coleman fat grafts or further isolated using mechanical shear force to prepare the SVF/ECM gel.In the control group,fat aspirates from patients undergoing liposuction were prepared into standard Coleman fat grafts,while in the experimental group,Coleman fat was further separated by mechanical shear force to prepare SVF/ECM gel.The fat retention rate,cell composition and phenotype of the two groups were compared.The fat retention rate,cell composition and phenotype of the two groups were compared.Results The retention rate of the SVF/ECM gel was significantly higher than that of the Coleman fat at 3,14,28,and 60 days following transplantation on the backs of nude mice.The viscosity of the fat was directly proportional to the shearing force.Although the mechanical isolation did not affect the total number of cells,it significantly decreased the number of living cells.Flow cytometry showed a greater number of mesenchymal stem cells,supra-adventitial adipose stromal cells,and adipose-derived stem cells but lower number of endothelial progenitor cells in the SVF/ECM gel than in the Coleman fat.Conclusion Mechanical isolation of fat can increase the pluripotency of adipocytes,which can improve graft retention in cell therapy.

关 键 词：血管基质片段 细胞外基质的凝胶状脂肪 科尔曼脂肪 剪切力 组织再生 干细胞 

分 类 号：R622.9[医药卫生—整形外科]