现代分析技术在天然产物立体异构体研究中的应用  

Application of modern analytical techniques to the study of stereoisomers of natural products

作　　者：李鹏娟 许洪波 杨远贵 LI Peng-juan;XU Hong-bo;YANG Yuan-gui(Shaanxi University of Traditional Chinese Medicine,Shaanxi Collaborative Innovation Center for the Industrialization of Traditional Chinese Medicine Resources,State Key Laboratory of Research and Development of Characteristic Qin Medicine Resources(Cultivation),Xianyang 712046,China)

机构地区：[1]陕西中医药大学,陕西省中药资源产业化协同创新中心,秦药特色资源研究开发国家重点实验室(培育),咸阳712046

出　　处：《药物分析杂志》2024年第10期1647-1654,共8页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金项目(82204516);陕西省科技厅一般项目-社会发展领域(2023-YBSF-281);陕西中医药大学博士科研启动金项目(306-1702032236)。

摘　　要：立体异构体是天然产物中的一种常见形式,不同构型异构体药理活性差异较大。高效分离解析立体异构体是研究天然产物药效物质亟待解决的问题之一。为了全面系统地综述立体异构体的分离解析方法,本文利用PubMed、Web of Science、Google Scholar、ACS、CNKI等在线数据库全面检索分离分析立体异构体的方法,系统归纳各方法的优缺点及适用范围。结果表明,立体异构体的分离方法主要有高效液相色谱法、二维液相色谱法、超临界流体色谱法、气相色谱法、毛细管电泳法等。此外,优先结晶法、膜分离和动力学拆分法等非色谱法也可用于立体异构体的拆分。常用的立体异构体解析方法有传统质谱法、离子淌度质谱法、核磁共振技术、单晶X-射线衍射法和光谱法等。通过对常见立体异构体分离分析方法的研究归纳总结,以期为天然产物中立体异构体的研究应用奠定基础。Stereoisomers are a common form in natural products, and the pharmacological activities of isomers with different configurations are various. Efficient separation and analysis of stereoisomers is one of the urgent problems for the study of pharmacodynamic substances of natural products. In order to comprehensively and systematically review the separation and analysis methods of stereoisomers, this review used online databases PubMed, Web of Science, Google Scholar, ACS, CNKI, and others to comprehensive search for the separation and analysis of the isomeric, and systematically summaries the advantages, disadvantages and scope of application. The results showed that the separation methods of stereoisomers mainly included high performance liquid chromatography, two-dimensional liquid chromatography, supercritical fluid chromatography, gas chromatography and capillary electrophoresis. In addition, non-chromatographic methods such as preferential crystallization, membrane separation and kinetic splitting can also be used for stereoisomers splitting. Commonly used stereoisomer resolution methods include conventional mass spectrometry, ion mobility mass spectrometry, nuclear magnetic resonance techniques, single-crystal X-ray diffraction and spectroscopy. The study of common stereoisomer separation and analysis methods is summarized with a view to laying a foundation for the research and application of stereoisomers in natural products.

关 键 词：立体异构体 天然产物 分离 解析 色谱法 非色谱法 

分 类 号：R917[医药卫生—药物分析学]