基于“脾-线粒体相关”研究运脾止泻方对腹泻幼鼠短链脂肪酸及能量代谢的影响  

Effect of Yunpi Zhixie Decoction on Short-Chain Fatty Acids and Energy Metabolism of Young Rats with Diarrhea Based on"Spleen-Mitochondria Correlation"

作　　者：王静[1] 万力生 倪新强 舒晴[3] WANG Jing;WAN Li-sheng;NI Xin-qiang;SHU Qing(Shenzhen Children′s Hospital,Guangzhou University of Traditional Chinese Medicine,Shenzhen 518038,China;The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine,Shenzhen 518000,China;Shenzhen Second Hospital,Shenzhen 518025,China)

机构地区：[1]广州中医药大学附属深圳市儿童医院,广东深圳518038 [2]广州中医药大学第四临床医学院,广东深圳518000 [3]深圳市第二人民医院,广东深圳518025

出　　处：《中药材》2024年第3期697-704,共8页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(82074492);广东省中医药局项目(20201295,20232152);广东省深圳市科技计划可持续发展专项项目(KCXFZ20201221173208023)。

摘　　要：目的:基于“脾-线粒体相关”理论,观察运脾止泻方对腹泻幼龄大鼠粪便短链脂肪酸、水液代谢及能量代谢的影响。方法:3周龄SD大鼠40只,按体质量随机分为正常对照组、模型组、蒙脱石散(0.18 g/kg)组和运脾止泻方(16 g/kg)组,每组10只。采用番泻叶药液(20 mL/kg)灌胃建立腹泻大鼠模型,连续造模14 d后,各给药组灌服相应药物,正常对照组和模型组灌胃相应体积0.5%羧甲基纤维素钠(CMC-Na)溶液,2次/d,连续1 w。记录大鼠腹泻情况、体质量、进食量的变化,统计对比大鼠稀便率、平均稀便级和腹泻指数;采集血清、粪便、结肠组织标本,检测粪便含水率,比色法检测血清D-木糖及结肠组织Na^(+)-K^(+)-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶水平,全自动血液分析仪检测大鼠红细胞计数、血红蛋白、红细胞比容,气相色谱法检测粪便短链脂肪酸含量;ELISA检测结肠组织ATP、AMP、ADP及血清MTL、GAS、SS、VIP水平;Western Blot检测结肠组织AQP3、AQP8、NHE1、NHE3蛋白表达。结果:与正常对照组比较,模型组大鼠体质量和进食量显著降低,粪便含水率、稀便率、平均稀便级、腹泻指数显著升高;血清D-木糖、VIP含量及红细胞计数、血红蛋白、红细胞比容显著降低,血清GAS、MTL、SS水平及粪便中乙酸、丙酸含量显著升高,粪便中丁酸含量及结肠组织ATP、ADP、Na^(+)-K^(+)-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶水平和AQP8、NHE3、NHE1蛋白表达显著降低,结肠组织AMP含量显著升高(P<0.05或P<0.01)。与模型组比较,运脾止泻方组大鼠体质量和进食量显著升高,粪便含水率、稀便率、平均稀便级、腹泻指数显著降低,血清D-木糖、VIP含量及红细胞计数、红细胞比容显著升高,血清GAS、SS水平及粪便中乙酸、丙酸含量显著降低,粪便中丁酸含量及结肠组织ATP、ADP、Na^(+)-K^(+)-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶水平和AQP8、AQP3、NHE3、NHE1蛋白表达显著升高,结肠组织AMP�Objective:To observe the effects of Yunpi Zhixie Decoction on short-chain fatty acids,aqueous metabolism and energy metabolism in faeces of young rats with diarrhea based on the theory of"spleen-mitochondria correlation".Methods:A total of 40 SD rats aged 3 weeks were randomly divided into normal control group,model group,Montmorillonite powder(0.18 g/kg)group and Yunpi Zhixie Decoction(16 g/kg)group according to body weight,with 10 rats in each group.The diarrhea rat model was established by intragastric administration of Sennae Folium solution(20 mL/kg).After 14 days of continuous modeling,the rats in drug administration groups were given the corresponding drugs,and the rats in normal control group and model group were given equal volume 0.5%sodium carboxymethylcellulose(CMC-Na)solution twice a day for 1 week.The changes of diarrhea,body mass and food intake of rats were recorded,and the rate of loose stool,average loose stool grade and diarrhea index were statistically compared.The serum,feces and colonic tissue samples were collected,fecal moisture content was measured.The levels of serum D-xylose and colonic tissue Na^(+)-K^(+)-ATPase and Ca^(2+)-Mg^(2+)-ATPase were determined by colorimetry,red blood cell count(RBC),hemoglobin(HGB)and hematocrit(HCT)were determined by automatic blood analyzer,and short-chain fatty acids contents in feces were detected by gas chromatography.The levels of ATP,AMP,ADP in colonic tissue and MTL,GAS,SS and VIP in serum were detected by ELISA.The AQP3,AQP8,NHE1,NHE3 protein expressions in colonic tissue were determined by Western Blot.Results:Compared with the normal control group,the body mass and food intake of rats in the model group were significantly decreased,and the fecal moisture content,rate of loose stool,average loose stool grade and diarrhea index were significantly increased,D-xylose,VIP contents in serum,RBC,HGB and HCT were significantly decreased,serum GAS,MTL,SS and feces acetic acid and propionic acid contents were significantly increased,the content of butyric

关 键 词：迁延性腹泻 运脾止泻方 短链脂肪酸 水液代谢 能量代谢 

分 类 号：R285.5[医药卫生—中药学]