胶体金免疫层析试纸条鉴别乳品中牛、羊源性成分的性能评价  

Performance Evaluation of Colloidal Gold Immunochromatographic Test Strips for Identification of Bovine-,Ovine-and Caprine-Derived Components in Dairy Products

作　　者：徐志鹏 泮秋立 肖晶 张爽 宋家升 王骏 XU Zhipeng;PAN Qiuli;XIAO Jing;ZHANG Shuang;SONG Jiasheng;WANG Jun(School of Public Health,Shandong Second Medical University,Weifang 261053,China;Key Laboratory of Supervising Technology for Meat and Meat Products,State Administration for Market Regulation,Shandong Institute for Food and Drug Control,Jinan 250101,China;China National Center for Food Safety Risk Assessment,Beijing 100022,China;Zhejiang Difurunsi Biotechnology Co.Ltd.,Hangzhou 310056,China)

机构地区：[1]山东第二医科大学公共卫生学院,山东潍坊261053 [2]山东省食品药品检验研究院,国家市场监督管理总局重点实验室(肉及肉制品监管技术),山东济南250101 [3]国家食品安全风险评估中心,北京100022 [4]浙江迪福润丝生物科技有限公司,浙江杭州310056

出　　处：《乳业科学与技术》2024年第6期37-42,共6页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：国家市场监督管理总局科技计划项目(2023MK078)。

摘　　要：依据《市场监管总局关于规范食品快速检测使用的意见》对市售现场快速鉴别乳品中牛、羊(绵羊、山羊)源性成分胶体金免疫层析产品性能进行评价,确定快检产品的适用范围、检出限、交叉反应率、灵敏度、假阴性率、假阳性率及与参比方法检测结果的符合率。结果表明:牛源性成分检测试纸条的适用范围为生牛乳,检出限为5%(V/V);羊源性成分检测试纸条的适用范围为生羊乳,检出限为0.3%(V/V)。牛、羊源性成分检测试纸条与恩诺沙星、磺胺二甲嘧啶、地塞米松、四环素、黄曲霉毒素M1均无交叉反应,方法灵敏度均为100%、假阳性率和假阴性率均为0%,与参比方法的检测结果符合率较高。综上,乳品中牛、羊源性成分胶体金免疫层析产品检测结果准确度较高,能用于掺假乳的现场快速筛查。According to the opinions of the State Administration for market Supervision on standardizing the use of rapid food detection,the performance of commercial colloidal gold immunochromatographic test strips for rapid in situ identification of bovine-,ovine-and caprine-derived ingredients in dairy products were evaluated in terms of application scope,detection limit,cross-reaction rate,sensitivity,false negative rate,false positive rate and coincidence rate with the results of the reference method.The results showed that the application scope of the test strip for bovine-derived ingredients was raw milk,and the detection limit was 5%(V/V);the application scope of the test strip for ovine-and caprine-derived ingredients was raw ovine and caprine milk,and the detection limit was 0.3%(V/V).Both test strips had no cross-reaction with enrofloxacin,sulfamethazine,dexamethasone,tetracycline or aflatoxin M1.The sensitivity,false positive rate and false negative rate of the test strips were 100%,0%and 0%,respectively.The coincidence rate with the results of the reference method was high.In conclusion,the colloidal gold immunochromatographic test strips have high accuracy,and can be used for rapid in situ screening of adulterated milk.

关 键 词：牛源乳品 羊源乳品 胶体金免疫层析 快速检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]