蒙药铁杆蒿对生菜的化感作用研究  

Allelopathy of Mongolian Medicine Artemisia Sacrorum Ledeb on Lettuce

作　　者：包国平 康高娃 王欢[1] 敖文达古拉 BAO Guoping;KANG Gaowa;WANG Huan;AO Wenda Gula(Mongolian Medical College,Inner Mongolia Minzu Univercity,Tongliao 028000,China;Tongliao Mongolian Medicine Orthopedic Hospital,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [2]通辽市蒙医整骨医院,内蒙古通辽028000

出　　处：《工业微生物》2024年第5期100-103,共4页Industrial Microbiology

基　　金：国家药品监管局中药(蒙药)质量控制重点实验室开放基金项目(编号:MDK2023030)。

摘　　要：对蒙药铁杆蒿进行初步化感作用实验,可以为蒙药铁杆蒿资源合理利用,以及为开发新型植物源除草剂提供科学依据。采用盘子法、三明治法测定蒙药铁杆蒿对生菜的化感作用,采用气质联用仪(GC-MS)测定主要化感物质。研究结果表明:铁杆蒿干燥叶对生菜幼苗根长和下胚轴的抑制率分别为40.5%、35%。铁杆蒿干燥叶浸提液10 mg/mL、50 mg/m L质量浓度时,对生菜幼根(抑制率:92%、98%)与下胚轴(抑制率:90%、100%)存在较显著的抑制作用。通过GC-MS分析,得出蒙药铁杆蒿主要成分为桉叶油醇。总之,蒙药铁杆蒿对生菜幼苗的生长具有显著的抑制作用,展现出作为新型植物源除草剂的潜力,也可为后续蒙药资源的合理开发利用提供依据。Conducted a preliminary allelopathy experiment on the Mongolian medicine Artemisia sacrorum Ledeb,in order to provide a scientific basis for the rational utilization of Mongolian medicine Artemisia sacrorum Ledeb resources and the development of new botanical herbicides.The plate dish-pack method and the sandwich method were used to determine the allelopathic effect of Mongolian medicine Artemisia sacrorum Ledeb on lettuce,and the main allelopathic substances were determined using gas chromatography-mass spectrometry(GC-MS).The inhibition rates of dried leaves of Mongolian medicine Artemisia sacrorum Ledeb on the root length and hypocotyl of lettuce seedlings were 40.5%and 35%.At the concentrations of 10 mg/mL and 50 mg/mL,the extract of dried Mongolian medicine Artemisia sacrorum Ledeb leaves showed significant inhibitory effects on lettuce seedling root(92%,98%)and hypocotyls(90%,100%).The main component analyzed by GC-MS was eucalyptol.The Mongolian medicine Artemisia sacrorum Ledeb has a significant inhibitory effect on the growth of lettuce seedlings,which can be used for the rational development and utilization of Mongolian medicine resources in the future,and has the potential as a new type of botanical herbicides.

关 键 词：化感作用 铁杆蒿 GC-MS 植物源除草剂 

分 类 号：S567.19[农业科学—中草药栽培] S636.2[农业科学—作物学]