肺炎支原体快速检测方法的建立(核酸检测)  

Establishment of a Rapid Detection Method for Mycoplasma Pneumoniae(Nucleic Acid Testing)

作　　者：卓明辉 ZHUO Minghui(Xiamen Baotai Biotechnology Co.,Ltd.,Xiamen 361000,China)

机构地区：[1]厦门宝太生物科技股份有限公司,福建厦门361000

出　　处：《工业微生物》2024年第5期131-133,共3页Industrial Microbiology

摘　　要：本研究根据肺炎支原体基因组中序列保守稳定的P1基因设计特异性引物和探针,建立高灵敏度和特异性的高效肺炎支原体快速核酸恒温扩增检测法。结果显示,该方法特异性强,能够准确检测肺炎支原体核酸,与肺炎衣原体、腺病毒及合胞病毒核酸均无交叉反应;灵敏度高,最低可检测10 copies/反应;检测时间短,最快5 min即可完成反应。用该检测方法对30例临床咽拭子样本进行检测,其检测结果与荧光定量PCR法相比,总体符合率达100%。本研究建立的LAMP恒温快速检测法操作简便,尤其适用于后续居家核酸快速检测产品的开发。The study designed specific primers and probes based on the P1 gene with conserved and stable sequence in the genome of Mycoplasma pneumoniae,and established a highly sensitive and specific rapid nucleic acid isothermal amplification detection method for Mycoplasma pneumoniae.The results showed that the method had strong specificity and can accurately detect the nucleic acid of Mycoplasma pneumoniae without cross reaction with the nucleic acid of Chlamydia pneumoniae,adenovirus,and syncytial virus.The method had high sensitivity,and could detect up to 10 copies/reaction.The detection time of this method was short,and the reaction can be completed in at least 5 minutes.The method was used to detect 30 clinical pharyngeal swab samples,and the overall conformity rate of the detection results was 100%compared with that of the quantitative real-time PCR method.The LAMP constant temperature rapid detection method established in this study was easy to operate and particularly suitable for the subsequent development of rapid home nucleic acid detection.

关 键 词：肺炎支原体 LAMP恒温 快速检测 

分 类 号：R446.5[医药卫生—诊断学]