PEG-6000模拟干旱胁迫对5个禾本科牧草品种幼苗抗氧化酶系统的影响  

Effects of PEG-6000 Simulated Drought Stress on Antioxidant Enzyme Systems of Five Varieties of Gramineous Forage Grass Seedlings

作　　者：刘杰 王玉辉 刘海聪[1] 罗黎鸣[3] 张彩峡[1] LIU Jie;WANG Yuhui;LIU Haicong;LUO Liming;ZHANG Caixia(College of Animal Science and Technology,Tibet Vocational Technology College,Tibet Lhasa 850030,China;College of Ecology and Environ-ment,Tibet University,Tibet Lhasa 850000,China;Institute of Agricultural,Tibet Academy of Agricultural and Animal Husbandry Sciences,Ti-bet Lhasa 850000,China)

机构地区：[1]西藏职业技术学院动物科学技术学院,西藏拉萨850030 [2]西藏大学生态环境学院,西藏拉萨850000 [3]西藏自治区农牧科学院农业研究所,西藏拉萨850000

出　　处：《西藏农业科技》2024年第4期52-56,共5页Tibet Journal of Agricultural Sciences

基　　金：中国科学院战略性先导科技专项子课题及西藏中央引导地方资金配套项目:西藏高寒区生态草牧业科技示范(XDA26050501,XZ202301YD0012C);校级科研课题(2020YBKT-08);专业带头人骨干教师队伍建设项目(0804F202305)。

摘　　要：为探究禾本科牧草苗期对干旱胁迫的响应,筛选出耐旱材料,选取5个禾本科牧草品种为研究对象,采用PEG-6000模拟干旱胁迫对禾本科牧草幼苗进行干旱处理,通过分析牧草幼苗的形态特征和抗氧化酶系统的活性变化,采用隶属函数法综合评价了5个禾本科牧草品种的抗旱性。研究发现,在干旱胁迫下,5个禾本科牧草品种的植株表型和生理特性均受到不同程度影响。不同禾本科牧草品种对干旱胁迫的适应机制是受多指标综合调控的。通过采用隶属函数综合分析,5个禾本科牧草品种的抗旱性强弱顺序依次为:小麦-高峰>燕麦-优牧1号>小黑麦-新麦1代>燕麦-麦多>燕麦-莫妮卡。牧草品种小麦-高峰能在干旱胁迫下保持较高的生长能力,以维持其在逆境中的生长发育,此外还能增加体内的抗氧化酶活性,通过消除活性氧来提高牧草对逆境的抵抗能力。综上所述,牧草品种小麦-高峰表现出更强的抗旱能力,可为培育抗旱型牧草品种提供材料基础,也为人工草地建植、草地生产力的提高和生态修复提供依据。In order to investigate the response of grass forage seedlings to drought stress and to screen out drought-tolerant materials,five grass forage varieties were selected as the research objects in this experiment,and PEG-6000 was used to simulate drought stress on grass forage seedlings,and the drought tolerance of the five grass forage varieties was comprehensively evaluated by affiliation function method through analyzing the morphology of the seedlings and the changes in the activities of antioxidant enzyme systems.It was found that under drought stress,the plant phenotypes and physiological characteristics of the five grass forage species were affected to different degrees.The adaptive mechanism of different grass forage species to drought stress was regulated by multiple indicators,and the order of drought resistance of the five grass forage species analyzed by using the affiliation function was as follows:Triticum aestivum-Gaofeng>Avena sativa-Youmu Number 1>Triticale-Xinmai Generation 1>Avena sativa-Maiduo>Avena sativa-Monika.The results showed that the forage variety Triticum aestivum-Gaofeng could maintain high growth capacity under drought stress to sustain its growth and development in adversity,in addition,it could increase the activity of antioxidant enzymes in the body,and improve the resistance of the forage to adversity by eliminating reactive oxygen species.In summary,the forage grass variety Triticum aestivum-Gaofeng showed stronger drought resistance,which can provide a material basis for cultivating drought-resistant forage grass varieties,as well as a basis for artificial grassland establishment,improvement of grassland productivity and ecological restoration.

关 键 词：干旱胁迫 禾本科牧草 幼苗 抗氧化酶系统 

分 类 号：S812.8[农业科学—草业科学]