基于网络药理学和体外实验探讨参芪扶正液对高糖诱导心肌损伤的保护作用  

Protective effect of Shenqi Fuzheng liquid on high glucose-induced myocardial injury using network pharmacology and in vitro experiment

作　　者：李琴 贺旭威 张勇刚 魏文滨 陈继铭 LI Qin;HE Xu-wei;ZHANG Yong-gang;WEI Wen-bin;CHEN Ji-ming(School of Pharmacy,Guangdong Medical University,Dongguan 523808,China;Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China)

机构地区：[1]广东医科大学药学院,广东东莞523808 [2]广东医科大学附属医院,广东湛江524001

出　　处：《广东医科大学学报》2024年第4期384-389,共6页Journal of Guangdong Medical University

基　　金：广东省中医药局中医药科研项目(20222101,20212098,20222105),广东省医学科学技术研究基金项目(A2020459),湛江市科技发展专项资金竞争性分配项目(2021A05063)。

摘　　要：目的探讨参芪扶正液对高糖诱导心肌损伤的潜在作用机制。方法从TCMSP、GeneCards和OMIM数据库中分别筛选参芪扶正液活性成分、作用靶点及心肌损伤基因,通过String数据库构建蛋白质相互作用(PPI)网络,使用Metascape数据库对交集基因进行GO、KEGG分析。H9c2心肌细胞分别用35 mmol/L葡萄糖或1、5 g/L参芪扶正液处理,MTT法检测细胞存活率,荧光染色检测细胞凋亡,Western blot检测PI3K/AKT表达。结果发现参芪扶正液495个作用靶点,心肌损伤靶点基因2148个,两者交集基因为227个,主要涉及内分泌抵抗、HIF-1、AMPK、PI3K/AKT等信号通路。参芪扶正液可增加心肌细胞存活,减少细胞凋亡,上调p-PI3K和p-AKT表达(P<0.05)。结论参芪扶正液可能通过PI3K/AKT信号减轻高糖诱导的心肌损伤。Objective To explore the potential mechanism of Shenqi Fuzheng liquid(SFL)on high glucose-induced myocardial injury(MI).Methods The active ingredients and targets of SFL,and MI genes were screened from TCMSP,GeneCards and OMIM databases,respectively.A PPI network was constructed using String database,and the intersection genes were analyzed by GO and KEGG using Metascape database.H9c2 myocardial cells were treated with 35 mmol/L glucose or adding with 1 and 5 g/L SFL.Survival rate,apoptosis and PI3K/AKT expression were detected by MTT,fluorescence staining and Western blot,respectively.Results There were 495 active ingredients of SFL,2148 genes of MI,and 227 intersecting genes between them.These intersecting genes were mainly involved in endocrine resistance,HIF-1,AMPK,PI3K/AKT signaling pathways.SFL increased survival rate,reduced apoptosis,and upregulated p-PI3K/p-AKT expression of injured myocardial cells.Conclusion SFL can alleviate high glucose-induced MI through PI3K/AKT signaling.

关 键 词：参芪扶正液 心肌损伤 网络药理学 PI3K/AKT信号通路 

分 类 号：R285[医药卫生—中药学]