大鼠脊髓损伤后MicroRNAs表达谱变化  

Changes of MicroRNAs expression profile after spinal cord injury in rats

作　　者：何燕飞[1] 唐瑷 汪晓宁[1] 徐东[1] 周英龙 帅勤 李龙文 李政[1] HE Yan-Fei;TANG Ai;WANG Xiao-Ning(Orthopedics Department,The People′s Hospital of Jinniu District,Chengdu City,Chengdu 610007,Sichuan,China)

机构地区：[1]成都市金牛区人民医院骨科,四川成都610007

出　　处：《吉林医学》2024年第11期2605-2609,共5页Jilin Medical Journal

基　　金：四川省科技厅科研课题资助[项目编号:21YYJC0321]。

摘　　要：目的:探究大鼠脊髓损伤后microRNAs表达谱变化。方法:将10只成年SD大鼠随机分为假手术组、模型组,建立脊髓损伤模型。术后采用Basso-Beattie-Bresnahan(BBB)量表进行运动功能评分,苏木精-伊红(HE)染色脊髓组织,通过Illumina高通量测序技术,对前期构建的cDNA文库进行RNA-Seq分析。将测序数据与高粱参考基因组对比后,以P<0.05为条件筛选出差异表达基因,通过GO、KEGG等数据库,对DEGs的功能以及参与的调控路径进行分析,并通过RT-qPCR验证RNA-Seq结果的可靠性。结果:假手术组与模型组BBB评分有显著性差异,HE染色观察到脊髓组织结构受损较为严重,脊髓损伤前后microRNAs表达有显著差异。结论:脊髓损伤许多基因表达失调。Objective To explore the changes of microRNAs expression profile after spinal cord injury in rats.Method Ten adult SD rats were randomly divided into sham operation group and model group to establish the model of spinal cord injury.After operation,the motor function was evaluated by Basso-Beattie-Bresnahan(BBB)scale,and the spinal cord tissue was stained with hematoxylin-eosin(HE).The cDNA library was analyzed by RNA-Seq by Illumina high-throughput sequencing technique.After comparing the sequencing data with the sorghum reference genome,the differentially expressed genes were screened under the condition of P<0.05.The function and regulatory pathway of DEGs were analyzed by GO,KEGG and other databases,and the reliability of RNA-Seq results was verified by RT-qPCR.Results There was significant difference in BBB score between sham operation group and spinal cord injury group.HE staining showed that the structure of spinal cord was seriously damaged,and there was significant difference in the expression of microRNAs before and after spinal cord injury.Conclusion Many genes are dysregulated in spinal cord injury.

关 键 词：脊髓损伤 表达谱 MICRORNAS 

分 类 号：R651.2[医药卫生—外科学]