大黄酸对CKD大鼠氧化应激作用的实验研究  

Experimental Study of the Impact of Rhein on Oxidative Stress in Rats with Chronic Idney Disease(CKD)

作　　者：马俊远 孔富康 张美乐 王刘阳 胡文慧 刘慧娟 Ma Junyuan;Kong Fukang;Zhang Meile;Wang Liuyang;Hu Wenhui;Liu Huijuan(Department of Medicine,Huanghe Science and Technology College,Zhengzhou City,Henan Province 450063)

机构地区：[1]黄河科技学院医学部,河南郑州450063

出　　处：《黄河科技学院学报》2024年第11期23-27,共5页Journal of Huanghe S&T College

基　　金：黄河科技学院创新教育示范课建设项目(JCXK202215);河南省大学生创新创业训练计划重点项目(S20231183408)。

摘　　要：评估大黄酸对由“腺嘌呤羧甲基纤维素钠混悬液”引发的雄性SD大鼠慢性肾病(CKD)模型的影响,并探讨其对血清中氧化应激标志物超氧化物歧化酶(Superoxide Dismutase,SOD)和丙二醛(MDA)的潜在改善作用。选取48只SPF级健康雄性SD大鼠随机分为2组:空白组12只、造模组36只,其中造模组采用腺嘌呤和羧甲基纤维素钠联合灌胃法构建CKD模型,造模结束后从空白组和造模组中分别随机选取6只大鼠,验证造模结果。造模成功后,造模组剩余大鼠随机分成5组,分别为模型组、厄贝沙坦组、大黄酸低浓度组、大黄酸中浓度组、大黄酸高浓度组,每组各6只。厄贝沙坦组、大黄酸低浓度组、大黄酸中浓度组、大黄酸高浓度组这4组大鼠连续灌胃给药21 d,且空白组和模型组给予与前4组灌胃量等量的蒸馏水。给药结束后,采用ELISA法测定SOD含量和MDA含量,通过HE染色观察各实验组肾脏组织病理改变。与空白组相比,模型组大鼠血清中SOD含量降低,MDA含量升高。与模型组相比,厄贝沙坦组及各浓度大黄酸组的SOD含量显著增高,MDA含量下降,差异有统计学意义。大黄酸对慢性肾病的氧化应激状态具有改善作用,这体现在提高SOD含量和降低MDA含量上,为大黄酸在未来临床上的应用提供了理论依据。The aim of this study was to evaluate the effects of rhein on chronic kidney disease(CKD)in male SD rats induced by"sodium adenine carboxymethyl cellulose suspension",and to investigate its potential improvement on serum markers of oxidative stress Superoxide Dismutase(SOD)and malondialdehyde(MDA).The methods were as follows:48 healthy male SD rats with SPF grade were randomly divided into 2 groups:blank group(12)and modeling group(36).CKD model was constructed in the modeling group using adenine and sodium carboxymethyl fiber combined gavage.After modeling,6 rats were randomly selected from the blank group and modeling group respectively to verify the modeling results.After the modeling was successful,the remaining rats in the modeling group were randomly divided into five groups:model group,irbesartan group,rhein low concentration group,rhein medium concentration group and rhein high concentration group,with 6 rats in each group.Irbesartan group,rhein low concentration group,rhein medium concentration group and rhein high concentration group were given continuous intragastric administration for 21 days,and blank group and model group were given the same amount of distilled water as the first four groups.After administration,SOD and MDA contents were measured by automatic biochemical detector,and pathological changes of renal tissue were observed by HE staining.Results:Compared with blank group,SOD content in serum of model group was decreased and MDA content was increased.Compared with the model group,SOD content in irbesartan group and rhein group was significantly increased,and MDA content was decreased,the difference was statistically significant.Conclusion:Rhein can improve the oxidative stress of chronic kidney disease,which is reflected in the increase of SOD content and the decrease of MDA content,which provides a theoretical basis for the future clinical application of rhein.

关 键 词：慢性肾脏病 大黄酸 SOD MDA 

分 类 号：R283[医药卫生—中药学]