神经纤毛因子1在骨折愈合中促进骨-血管偶联的作用  

Role of neuropilin 1 in promoting angiogenesis-osteogenesis coupling during fracture healing

作　　者：郑丽 丁一桁 李新浩 文泽开 姜炳正 林雪霞 Zheng Li;Ding Yiheng;Li Xinhao;Wen Zekai;Jiang Bingzheng;Lin Xuexia(Xingtai Medical College,Xingtai 054000,Hebei Province,China;Hebei Key Lab of Laboratory Animal Science,Department of Experimental Animal Science of Hebei Medical University,Shijiazhuang 050017,Hebei Province,China)

机构地区：[1]邢台医学院,河北省邢台市054000 [2]河北省实验动物学重点实验室,河北医科大学实验动物学部,河北省石家庄市050017

出　　处：《中国组织工程研究》2025年第21期4576-4583,共8页Chinese Journal of Tissue Engineering Research

基　　金：河北省重点研发计划项目(223777133D),项目负责人:郑丽。

摘　　要：背景:神经纤毛因子1在骨折愈合过程中发挥重要作用,尤其是在促进骨-血管偶联方面。骨-血管偶联是骨折愈合过程中的关键事件之一,包括血管(尤其是H型血管)、新骨形成以及它们之间的关联。目的:回顾神经纤毛因子1在骨折愈合过程中促骨-血管偶联作用的研究进展。方法:在中国知网、PubMed数据库检索1982年1月至2024年4月发表的文献,以“Neuropilin-1,neuropilin 1,NRP1,neuropilin-1,NRP-1,Neuropilin 1,Nrp1,Nrp-1”为检索词,从中选择与骨折愈合、H型血管、动脉、骨细胞、骨-血管偶联关系紧密的文献43篇,增加手工检索的文献24篇,最终得到67篇文献。结果与结论:(1)在骨折愈合过程中骨-血管偶联的关键事件包括血管生成、新骨形成以及它们之间的关联,其中H型血管生成是骨折愈合过程中的关键事件之一,H型血管由血管内皮细胞以及周细胞组成;新骨形成主要是由成骨细胞、破骨细胞以及软骨细胞等骨细胞共同完成的;(2)神经纤毛因子1可以通过血管内皮生长因子和血小板衍生生长因子促进内皮细胞迁移、稳定,增强周细胞稳定性及其与内皮细胞之间的关联,从而促进血管再生;(3)神经纤毛因子1通过促进成骨细胞和软骨细胞形成、抑制破骨细胞生成来促进骨再生;(4)神经纤毛因子1可能是血管和骨偶联的关键因子之一;(5)局部应用神经纤毛因子1及其生物制剂,或将神经纤毛因子1、纳米载药系统以及高分子智能聚合材料等生物工程材料融合,为骨折治疗提供了新思路,有着广阔的应用前景。BACKGROUND:Neuropilin 1 plays a critical role in fracture healing,especially in osteogenesis-angiogenesis coupling.Osteogenesis-coupled angiogenesis for fracture healing,including angiogenesis and bone formation(especially H-type vessel formation)and their association.OBJECTIVE:To review the research progress of neuropilin 1 in promoting osteogenesis-coupled angiogenesis during fracture healing.METHODS:The literature search was performed in the PubMed and Chinese National Knowledge Infrastructure databases for articles published from January 1982 to April 2024 using the search terms“Neuropilin-1,neuropilin 1,NRP1,neuropilin-1,NRP-1,Neuropilin 1,Nrp1,Nrp-1.”We identified 43 articles associated with fracture healing,H-type vessels,arteries,bone cells,and angiogenesis-osteogenesis coupling.Moreover,24 articles were searched manually.Finally,67 papers were included.RESULTS AND CONCLUSION:(1)Angiogenesis,osteoblastogenesis,and their association were critical for angiogenesis-osteogenesis coupling during fracture healing.Especially H-type vessel formation was critical during fracture healing.H-type vessels consisted of endothelial cells and pericytes.Osteoblasts,osteoclasts,chondrocytes,and other skeletal cells were critical contributors to new bone formation.(2)Neuropilin 1 enhanced endothelial cell migration and stabilization,increased stability of endothelial cells and pericytes,and strengthened the link of endothelial cells and pericytes to initiate angiogenesis by vascular endothelial growth factor and platelet-derived growth factor.(3)Neuropilin 1 promoted bone regeneration by suppressing osteoclastogenesis and enhancing osteoclasts and chondrocyte formation.(4)Neuropilin 1 was a key factor in angiogenesis-osteogenesis coupling.(5)Topical application of neuropilin 1 and relative biologics,and combination neuropilin 1,nano drug-loading systems,and polymerized smart materials provided a new idea for treatment of fractures and hold great promise for extensive applications.

关 键 词：神经纤毛因子1 骨折愈合 骨-血管偶联 H型血管 内皮细胞 周细胞 成骨细胞 破骨细胞 软骨细胞 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R683