具有类漆酶活性的铜基纳米酶检测及降解酚类化合物的研究进展  

Progress on Copper-Based Nanozymes with Laccase-Like Activity in Phenol Detection and Degradation

作　　者：杜明月 陈淑 王亚雯 丁龙华 DU Mingyue;CHEN Shu;WANG Yawen;DING Longhua(Institute for Advanced Interdisciplinary Research(iAIR),University of Jinan,Jinan 250022,China;School of Bioengineering,Shandong Polytechnic,Jinan 250104,China)

机构地区：[1]济南大学前沿交叉科学研究院,山东济南250022 [2]山东职业学院生物工程学院,山东济南250104

出　　处：《铜业工程》2024年第5期79-87,共9页Copper Engineering

基　　金：国家自然科学基金项目(51802118);山东省自然科学基金项目(ZR2023MB126,ZR2023QB298)资助。

摘　　要：酚类物质是一类高毒性的有机污染物,对生态安全和人类健康构成严重威胁。因此,检测和去除酚类物质至关重要。天然漆酶作为一种多酚氧化酶,能够高效地催化多酚氧化生成水,具有绿色高效的多功能生物催化剂特性,在生物传感和污染物降解领域具有重要应用价值。然而,天然漆酶的成本高、稳定性差且难以回收利用,这些问题限制了其实际应用。为解决上述问题,研究者开发了纳米酶,这是一种具有类似天然酶催化特性的功能材料,能够弥补天然酶的不足。受天然漆酶活性中心含有4个配位铜原子的启发,设计并合成了铜基纳米酶材料。本文主要介绍了漆酶活性的评价方法,以及由不同有机化合物配体(有机小分子、核苷酸和氨基酸等)组成的铜基有机杂化纳米酶,并探讨了配位原子和配位环境对漆酶活性的影响。同时,总结了铜基有机杂化材料在酚类物质检测和降解方面的应用进展,并展望了具有类漆酶活性的铜基有机杂化材料的发展趋势。Phenolic compounds,a highly toxic group of organic pollutants,pose significant risks to ecological security and human health.Their detection and elimination is crucial.Laccase,a polyphenol oxidase,efficiently catalyzes polyphenol oxidation to produce water,showing green and efficient biocatalytic properties valuable in biosensing and pollutant degradation.Practical use of laccase is hindered by high cost,poor stability,and recycling challenges.Nanozymes,functionally similar to natural enzymes,can address these drawbacks.Copper-based mimetic enzyme materials,inspired by laccase's copper-containing active center,have emerged as effective laccase mimics.This article introduces laccase activity evaluation methods and discusses copper-based organic hybrid nanozymes with various ligands such as small molecules,nucleotides,and amino acids,exploring their impact on laccase activity.Progress in using copper-based organic hybrid materials for phenolic compound detection and degradation is summarized,along with forecasted trends for developing copper-based organic hybrids with laccase-like activity.

关 键 词：类漆酶活性 铜基有机杂化材料 纳米酶 多酚物质检测 催化活性 

分 类 号：O643[理学—物理化学]