丹参通络解毒汤的急性毒性研究  

Study on the Acute Toxicity of Danshen Tongluo Jiedu Decoction

作　　者：刘泳钊 颜梦凡 李彩云 李鑫辉 LIU Yong-zhao;YAN Meng-fan;LI Cai-yun;LI Xin-hui(School of Chinese Medicine,Hunan University of Traditional Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学中医学院,湖南长沙410208

出　　处：《时珍国医国药》2024年第11期2513-2517,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(82074392);湖南省自然科学基金区域联合基金项目(2023JJ50036);湖南省中医药科研项目重点课题(A2023013)。

摘　　要：目的研究丹参通络解毒汤(DSTLJD)的急性毒性,为临床用药安全提供实验依据。方法40只昆明小鼠随机分为DSTLJD组、对照组,DSTLJD组给予最大给药量160g/kg灌胃,2次/d,对照组灌胃等量生理盐水,观察小鼠一般状况,记录摄入量及体重变化,14d后解剖小鼠观察脏器并计算指数,用生化试剂盒测定血清中肝肾功能相关指标,HE染色观察主要脏器的病理学变化。结果小鼠最大给药量相当于成人临床用药量116倍,灌胃后小鼠精神状态、自发活动等一般状况无明显异常改变,无死亡。与对照组比较,DSTLJD组部分小鼠出现拉稀症状,摄食量减少、体重增加延缓(P<0.05),饮水量无显著差异(P>0.05);解剖小鼠后发现心、肝、脾、肺、肾、胃、大肠、小肠等脏器表观均无明显的病理改变,DSTLJD组小鼠脏器指数与对照组比较,差异无统计学意义(P>0.05);与对照组相比,DSTLJD组雌、雄小鼠血清谷丙转氨酶、谷草转氨酶、碱性磷酸酶、总胆红素、肌酐均无显著差异(P>0.05),尿素氮含量明显降低(P<0.05);HE染色观察肝、心、脾、肺、肾、胃、大肠、小肠等脏器均无明显的病理学变化。结论丹参通络解毒汤无明显的急性毒性,药物安全性好,临床用药安全可靠。Objective To study the acute toxicity of Danshen Tongluo Jiedu Decoction(DSTLJD)and provide experimental basis for clinical medication safety.Methods Forty Kunming mice were randomly divided into a DSTLJD group and a control group.The DSTLJD group was given a maximum dose of 160g/kg by gavage twice a day,while the control group was given an equal amount of physiological saline by gavage.The general condition of the mice was observed,and the intake and weight changes were recorded.After 14 days,the organs of the mice were dissected and the index was calculated.The liver and kidney function related indicators in serum were measured using a biochemical reagent kit,and the pathological changes of the main organs were observed using HE staining.Results The maximum dose administered to mice was 116 times the clinical dose used in adults.After gavage,there were no significant abnormal changes in the mental state,spontaneous activity,and other general conditions of the mice,and there was no death.Compared with the control group,some mice in the DSTLJD group showed symptoms of diarrhea,decreased dietary intake and delayed weight gain(P<0.05),while there was no significant difference in water consumption(P>0.05);After dissecting the mice,it was found that there were no significant pathological changes in the appearance of organs such as the heart,liver,spleen,lungs,kidneys,stomach,large intestine,and small intestine.The organ index of the DSTLJD group mice was not significantly different from the control group(P>0.05);Compared with the control group,there was no significant difference in serum alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase,total bilirubin,and creatinine between female and male mice in the DSTLJD group(P>0.05),and the urea nitrogen content was significantly reduced(P<0.05);HE staining showed no significant pathological changes in organs such as liver,heart,spleen,lung,kidney,stomach,large intestine,and small intestine.Conclusion Danshen Tongluo Jiedu Decoction has no obvious acute

关 键 词：丹参通络解毒汤 急性毒性 安全性 清营解毒 

分 类 号：R285.5[医药卫生—中药学]