精氨酸改性生物质炭固定阴沟肠杆菌TMX-6的优化及降解噻虫嗪的效果  

Optimization of immobilization of Enterobacter cloacae TMX-6 by arginine-modified biochar and its degradation effect on thiamethoxam

作　　者：曹瑶瑶 葛静[1] 生弘杰 冯发运 万群[1] 马丽雅 张雷刚[1] 余向阳[1] CAO Yaoyao;GE Jing;SHENG Hongjie;FENG Fayun;WAN Qun;MA Liya;ZHANG Leigang;YU Xiangyang(Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base,Ministry of Science and Technology,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院农产品质量安全与营养研究所/江苏省食品质量安全重点实验室—省部共建国家重点实验室培育基地,江苏南京210014

出　　处：《江苏农业学报》2024年第9期1749-1757,共9页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(32302406、42007133、31772197);江苏省农业科技自主创新基金项目[CX(22)3074]。

摘　　要：噻虫嗪的广泛使用对生态环境和人体健康造成直接或潜在的安全隐患。为实现残留噻虫嗪的快速降解,本研究在精氨酸改性生物质炭(Arg@BC)的基础上,通过单因素试验和正交试验,明确Arg@BC与阴沟肠杆菌TMX-6制备Arg@BC/TMX-6炭基菌剂的优化方案,并对不同剂型的TMX-6对噻虫嗪的降解能力进行比较。结果表明,Arg@BC中精氨酸含量与Arg@BC/TMX-6中TMX-6单位固定量呈正相关关系,对生物质炭进行精氨酸改性可提高生物质炭对带负电细菌的捕获性能。Arg@BC/TMX-6制备的最优条件为Arg@BC质量浓度50 g/L、精氨酸与生物质炭重量比5.0∶10.0、固定化12 h。Arg@BC/TMX-6对噻虫嗪降解率(50.69%)高于相当量的Arg@BC与TMX-6的降解率之和,即精氨酸改性生物质炭再固定TMX-6有利于提高TMX-6对噻虫嗪的降解能力。本研究结果为进一步利用炭基材料固定降解微生物应用于农药污染土壤的修复提供了理论依据。The widespread use of thiamethoxam poses a direct or potential safety hazard to the ecological environment and human health.In order to achieve the rapid degradation of residual thiamethoxam,on the basis of arginine modified biochar(Arg@BC),the optimal preparation scheme of carbon-based microbial agent Arg@BC/TMX-6 prepared by Arg@BC and Enterobacter cloacae TMX-6 was determined by single factor and orthogonal experiments,and the degradation ability of different formulations of TMX-6 to thiamethoxam was compared.The results showed that the arginine content in Arg@BC was positively correlated with the fixed amount of TMX-6 in Arg@BC/TMX-6.Arginine modification could improve the capture performance of biochar to negatively charged bacteria.The optimal conditions for the preparation of Arg@BC/TMX-6 were as follows:the mass concentration of Arg@BC was 50 g/L,the weight ratio of arginine to biochar was 5.0∶10.0,and the immobilization time was 12 h.The degradation rate(50.69%)of thiamethoxam by Arg@BC/TMX-6 was higher than the sum of the degradation rates of Arg@BC and TMX-6,that is,arginine-modified biochar re-immobilized TMX-6 was beneficial to improve the degradation ability of TMX-6 to thiamethoxam.The results of this study provide a theoretical basis for the further use of carbon-based materials to immobilize degrading microorganisms for the remediation of pesticide-contaminated soil.

关 键 词：噻虫嗪 精氨酸 生物质炭 农药残留 农药降解 

分 类 号：X592[环境科学与工程—环境工程]